CELL IMAGING

细胞成像

• 批准号: 7137090
• 负责人: MICHAEL H NATHANSON
• 金额: $ 13.01万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7137090
• 关键词: bioimaging /biomedical imaging; biomedical facility; calcium flux; cell biology; computer data analysis; confocal scanning microscopy; image processing; immunofluorescence technique; light microscopy; liver cells; protein localization

The purpose of the Cell Imaging Core is to provide access to equipment and technical expertise for confocal microscopy, combined confocal and multi-photon microscopy, and image processing and analysis. The range of experiments that will take advantage of this core include immunofluorescence, imaging of GFP, DsRed, RFP, and other organelle- and protein-specific dyes, time-lapse confocal microscopy, calcium imaging, and confocal imaging combined with multi-photon flash photolysis of caged compounds. This Core will take advantage of the medical school's existing Center for Cell and Molecular Imaging in order to provide these services. Equipment in the core will include three Zeiss LSM 510 confocal microscopes and two PCs for off-line image processing and data analysis. The first of the three confocal microscopes is a standard point-scanning system with three detection channels, while the second is equipped with a META detector for spectral deconvolution, and the third has nonlinear optics, non-descanned detectors, and is equipped with a Spectra-Physics 10 watt tunable femtosecond-pulsed titaniurrrsapphire laser for multiphoton excitation. This Core also will provide a Research Support Specialist who is experienced in confocal and multi-photon microscopy, plus a Research Biotechnologist who has experience with lasers, optics and microscopy design. The Research Support Specialist will assist members of each project with all experiments requiring the use of this core facility, while the Research Biotechnologist will work with project members to design new optical imaging methods and techniques that relate to the research aims of the PPG.

细胞成像核心的目的是为细胞提供设备和技术专业知识 共焦显微镜、共焦和多光子显微镜相结合，以及图像处理和分析。 利用该核心的实验范围包括免疫荧光、成像 GFP、DsRed、RFP 和其他细胞器和蛋白质特异性染料、延时共聚焦显微镜、钙 成像，以及与笼状化合物的多光子闪光光解相结合的共焦成像。这个核心 将利用医学院现有的细胞和分子成像中心 提供这些服务。核心设备将包括三台 Zeiss LSM 510 共焦显微镜和 两台PC用于离线图像处理和数据分析。三台共焦显微镜中的第一台是 标准点扫描系统具有三个检测通道，第二个配备 META 用于光谱反卷积的探测器，第三个具有非线性光学器件、非退扫描探测器，并且是 配备 Spectra-Physics 10 瓦可调谐飞秒脉冲钛蓝宝石激光器，用于多光子 励磁。该核心还将提供一名在共聚焦方面经验丰富的研究支持专家 和多光子显微镜，再加上一位在激光、光学和 显微镜设计。研究支持专家将协助每个项目的成员处理所有问题 需要使用该核心设施的实验，而研究生物技术专家将与项目合作 成员设计与研究目标相关的新光学成像方法和技术 PPG。