CORE--TISSUE AND CELL MOLECULAR ANALYSIS

核心——组织和细胞分子分析

• 批准号: 6989941
• 负责人: Wilma Lingle
• 金额: $ 6.56万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989941
• 关键词: biomedical facility; colorimetry; computer data analysis; digital imaging; fluorescent dye /probe; health science research support; histology; image processing; immunocytochemistry; in situ hybridization; laser capture microdissection; molecular oncology; neoplasm /cancer; neoplastic cell; pathology; protein localization; tissue /cell preparation

The Tissue and Cell Molecular Analysis Shared Resource provides research service for pathology, immunohistochemistry, in situ hybridization, digital image analysis, laser capture microdissection, and preparation of tissues for subsequent protein extraction for members of the Mayo Clinic Cancer Center. During 2002, this resource was used by more than 70 investigators at the Mayo Clinic and supported cancer research funded by more than 50 NCI-recognized grants. Several areas of service are being expanded and developed in the TACMA Shared Resource: 1) digital image analysis performed using a Bliss "Virtual Microscopy" microscope and computer system from Bacus Laboratories will be available across all three Mayo sites via the Mayo intranet and shared server space, 2) technical protocols have been developed for detection of phosphorylation-state specific proteins for analysis of signaling pathways in paraffin embedded tissues, 3) protocols for simultaneous detection of two antigens are being developed, 4) the feasibility of using Zenon TM direct label probes for IHC is being investigated, 5) fluorescent probes are being used for subcellular localization of antigens in paraffin embedded tissues at a resolution much greater than is possible with calorimetric IHC detection chemistries that use brightfield microscopy. The services provided by the TACMA Shared Resource have been vital to cancer research at the Mayo Clinic since the inception of the MCCC. By expanding technical services and developing protocols for new probes, the TACMA resource can help Mayo investigators lead the way into the future of cancer research.

组织和细胞分子分析共享资源为梅奥诊所癌症中心的成员提供病理学、免疫组织化学、原位杂交、数字图像分析、激光捕获显微切割以及随后蛋白质提取的组织准备等研究服务。 2002 年期间，该资源被 Mayo Clinic 的 70 多名研究人员使用，并支持由 50 多项 NCI 认可的赠款资助的癌症研究。 TACMA 共享资源正在扩展和开发多个服务领域：1) 使用 Bacus 实验室的 Bliss“虚拟显微镜”显微镜和计算机系统进行的数字图像分析将通过 Mayo 内联网和共享服务器在所有三个 Mayo 站点提供空间，2) 已开发出用于检测磷酸化状态特异性蛋白质的技术方案，用于分析石蜡包埋中的信号通路 组织，3) 正在开发同时检测两种抗原的方案，4) 正在研究使用 Zenon TM 直接标记探针进行 IHC 的可行性，5) 荧光探针正在用于石蜡包埋组织中抗原的亚细胞定位分辨率远高于使用明场显微镜的量热 IHC 检测化学方法。自 MCCC 成立以来，TACMA 共享资源提供的服务对梅奥诊所的癌症研究至关重要。通过扩大技术服务和开发新探针的方案，TACMA 资源可以帮助梅奥研究人员引领癌症研究的未来。