TECNIA 12 BioTWIN Transmission Electron Microscope

TECNIA 12 BioTWIN 透射电子显微镜

• 批准号: 6577399
• 负责人: GUIDO A ZAMPIGHI
• 金额: $ 49.41万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6577399
• 关键词: biomedical equipment purchase; transmission electron microscopy

DESCRIPTION (provided by applicant): A group of ten faculty sharing a common interest in the structure of functional ion channels and transporters and with an extensive record of past and current collaborations request funds to replace an over twenty years old Zeiss 12C electron microscope with a new Tecnai 12 Bio TWIN Transmission Electron Microscopy System. The selection of this system is based on several features critical to the work currently underway in the laboratories of the participating faculty. First, the Tecnai 12 will upgrade tilting capabilities by providing a eucentric stage with +80-degree tilts. This capability would provide high tilt views of channels and transporters embedded in lipid bilayers, which are critical to resolve the "missing cone" problem arising from imaging samples using "random conical tilt" geometry. Second, the Tecnai 12 will be equipped with a CCD camera. This upgrade will eliminate darkroom related work and will greatly speed up data collection. Third, the Tecnai 12 will be integrated into a network for export of data in the laboratories of the participating faculties. This capability will create a high trough put system for data analysis. Fourth, the Tecnai 12 will be equipped with software for automated data collection, written specifically for this system. Finally, the Tecnai 12 has an excellent illumination system and low-dose device. The improved capabilities of the requested system will impact the research programs of 10 faculties in the UCLA School of Medicine and the College of Letters and Sciences currently studying the structure and function of ion channels and transporters.

描述（由申请人提供）： 一个由 10 名教师组成的小组，对功能离子通道和转运蛋白的结构有着共同的兴趣，并且在过去和当前的合作中有广泛的记录，请求资金用新的 Tecnai 12 Bio TWIN 透射电子显微镜替换已有 20 多年历史的 Zeiss 12C 电子显微镜显微镜系统。该系统的选择基于对参与教师实验室当前正在进行的工作至关重要的几个功能。首先，Tecnai 12 将通过提供 +80 度倾斜的 eucentric 平台来升级倾斜功能。这种能力将提供嵌入脂质双层中的通道和转运蛋白的高倾斜视图，这对于解决使用“随机圆锥倾斜”几何形状对样品进行成像时出现的“缺失圆锥”问题至关重要。其次，Tecnai 12将配备CCD摄像头。此次升级将消除暗室相关工作，并将大大加快数据收集速度。第三，Tecnai 12 将被集成到参与院系实验室的数据导出网络中。此功能将为数据分析创建一个高槽位系统。第四，Tecnai 12 将配备专门为此系统编写的自动数据收集软件。最后，Tecnai 12 拥有出色的照明系统和低剂量装置。所需系统的改进功能将影响加州大学洛杉矶分校医学院和文学与科学学院的 10 个院系目前研究离子通道和转运蛋白的结构和功能的研究项目。