STRUCTURE AND FUNCTION OF LENS CHANNELS

镜头通道的结构和功能

• 批准号: 6518312
• 负责人: GUIDO A ZAMPIGHI
• 金额: $ 28.01万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-08-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6518312
• 关键词: Xenopus oocyte; aging; cataract; cell cell interaction; electron microscopy; electrophysiology; freeze etching; gap junctions; immunocytochemistry; intracellular transport; lens; lens proteins; membrane channels; membrane permeability; membrane transport proteins; protein structure function; water channel; western blottings

DESCRIPTION (adapted from the abstract)This proposal is to study the molecular properties and location of the "aquaporin" and "connexin" channels in the lens. Lens aquaporin channels (AQP1 and MIP) mediate and regulate water permeability, a critical component of the hypothesized lens internal circulatory system. Lens connexin channels (Cx43, Cx46 and Cx50) are hypothesized to conduct and direct this circulation to be inward at the anterior and posterior poles and outward at the equator. This proposal aims to locate these channels in different regions of the lens, to quantify their density and to determine how changes due to aging and some naturally occurring mutations can lead to cataract formation. To identify the proteins, this proposal will use freeze fracture label electron microscopy. To study their functional properties, expression in Xenopus laevis oocytes will be used to allow measurements of molecular properties, electrolyte and nonelectrolyte fluxes and water permeability.

描述（根据摘要改编）该建议是研究镜头中“ Aquaporin”和“连接蛋白”通道的分子特性和位置。透镜水通道通道（AQP1和MIP）介导并调节水的渗透性，这是假设透镜内部循环系统的关键组成部分。假设透镜连接蛋白通道（CX43，CX46和CX50）进行并指导该循环在前杆和后杆上，并在赤道向外。该建议旨在将这些通道定位在镜头的不同区域，以量化其密度，并确定由于衰老和某些天然发生的突变而导致的变化如何导致白内障形成。为了识别蛋白质，该建议将使用冻结标签电子显微镜。为了研究其功能特性，将使用Laevis卵母细胞中的表达来测量分子特性，电解质和非电解质通量以及渗透性。