JEOL 1230 TEM and BOC Edwards Vacuum Evaporator

JEOL 1230 TEM 和 BOC Edwards 真空蒸发器

• 批准号: 6733441
• 负责人: MICHAEL J. WELSH
• 金额: $ 38.34万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6733441
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; evaporation; tissue /cell preparation; transmission electron microscopy

DESCRIPTION (provided by applicant): This proposal requests funds for a JEOL 1230 Transmission Electron Microscope (TEM) and a BOC Edwards Auto306 Vacuum Evaporator. These instruments will be located in the University of Iowa Central Microscopy Research Facility. The JEOL 1230 is being requested because the facility currently has just one TEM, a Hitachi H-7000 purchased with university funds in 1987. During the past 12 months, 78 faculty labs have used this instrument to support their research. 60 of these investigators have NIH funded research grants. 8 students each semester are enrolled in an Electron Microscopy Techniques course and use this instrument. Investigators have to sign up for the Hitachi TEM 2 to 3 weeks in advance. A number of operators are using the TEM during evenings and weekends. There is no guarantee that the instrument will be functional at a scheduled period because of unexpected downtime for maintenance. This results from a combination of very heavy use, the age of the Hitachi, which is a design introduced in 1981, and the low level of expertise of service personnel. Investigators also need low dose imaging, automated stage controls, tomography, digital imaging and Cryo-TEM to study their samples. The Hitachi TEM lacks all of these modern capabilities. The BOC Edwards Vacuum Evaporator will replace a 30 year old Varian unit in the facility. Repair parts have been unavailable for over 15 years; it suffers from poor vacuum and inconsistent evaporation of carbon and metals. It is critical that carbon coated grids for thin-sections and carbon thin films for negative staining, metal coating and Cryo-TEM be very-thin and low contrast. The JEOL TEM and the BOC Edwards evaporator will be conveniently located in room 84 and 92 respectively in the Eckstein Medical research Building as part of the University of Iowa Central Microscopy Research Facility. Administration and maintenance, as well as high-level technical assistance and training, will be provided by experienced personnel within the facility. Institutional support is strong with $61,234.00 being provided towards purchase of the instruments, $55,133.00 in full salary support for a research assistant and $10,000.00 to partially fund the annual service contract for the JEOL TEM on a continuing basis. Welsh will study airway epithelial cell biology, airway infections and gene transfer. Apicella plans to investigate the pathogenesis of bacteria. Dailey will examine synapse development and injury in brain. McCray will be involved with gene transfer using lentiviral vectors. Tobacman will study the structure of short actin filaments. Zabner will examine the morphology of Adeno-associated virus.

描述（由申请人提供）：该提案请求资金购买 JEOL 1230 透射电子显微镜 (TEM) 和 BOC Edwards Auto306 真空蒸发器。这些仪器将位于爱荷华大学中央显微镜研究设施内。之所以需要 JEOL 1230，是因为该设施目前只有一台 TEM，即 1987 年用大学资金购买的一台 Hitachi H-7000。在过去 12 个月中，78 个教职员工实验室使用了该仪器来支持他们的研究。其中 60 名研究人员获得了 NIH 资助的研究经费。每学期有 8 名学生注册电子显微镜技术课程并使用该仪器。研究人员必须提前 2 至 3 周报名参加日立 TEM。许多操作员在晚上和周末使用 TEM。由于维护意外停机，无法保证仪器在预定时间内正常运行。这是由于使用频繁、日立机（1981 年推出的设计）的使用年限以及服务人员的专业知识水平低等综合因素造成的。研究人员还需要低剂量成像、自动阶段控制、断层扫描、数字成像和冷冻透射电镜来研究他们的样本。 Hitachi TEM 缺乏所有这些现代功能。 BOC Edwards 真空蒸发器将取代该设施中已有 30 年历史的瓦里安设备。维修零件已超过 15 年无法使用；它的真空度较差，碳和金属的蒸发不一致。至关重要的是，用于薄片的碳涂层网格和用于负染色、金属涂层和冷冻透射电镜的碳薄膜必须非常薄且对比度低。 JEOL TEM 和 BOC Edwards 蒸发器将分别位于 Eckstein 医学研究大楼的 84 号和 92 号房间，作为爱荷华大学中央显微镜研究设施的一部分。管理和维护以及高水平的技术援助和培训将由设施内经验丰富的人员提供。机构支持非常强大，其中 61,234.00 美元用于购买仪器，55,133.00 美元为研究助理提供全额工资支持，10,000.00 美元用于持续为 JEOL TEM 的年度服务合同提供部分资金。威尔士将研究气道上皮细胞生物学、气道感染和基因转移。阿皮塞拉计划研究细菌的发病机制。戴利将检查大脑中突触的发育和损伤。麦克雷将参与使用慢病毒载体的基因转移。 Tobacman 将研究短肌动蛋白丝的结构。扎布纳将检查腺相关病毒的形态。

项目成果

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• 通讯作者: Grose C

Cardiac Med1 deletion promotes early lethality, cardiac remodeling, and transcriptional reprogramming.

• DOI: 10.1152/ajpheart.00728.2016
• 发表时间: 2017-04
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• 作者: Kathryn M. Spitler;Jessica M. Ponce;G. Oudit;D. Hall;Chad E. Grueter

A Drosophila Model of Epidermolysis Bullosa Simplex.

• DOI: 10.1038/jid.2015.129
• 发表时间: 2015-08
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• 通讯作者: Magin TM

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• 影响因子: 13.3
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• 通讯作者: Lira, Vitor A.