JEOL Transmission Electron Microscope

JEOL 透射电子显微镜

• 批准号: 6731447
• 负责人: KARL WEISGRABER
• 金额: $ 46.2万
• 依托单位: J. DAVID GLADSTONE INSTITUTES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-15 至 2006-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731447
• 关键词: biomedical equipment purchase; charge coupled device camera; computers; transmission electron microscopy

DESCRIPTION (provided by applicant): The objective of this shared equipment grant is to obtain a JEOL JEM-1230 transmission electron microscope, a specimen rotation holder, a GATAN Ultrascan 1000 CCD camera, and a dedicated computer, for the community of biomedical investigators at the J. David Gladstone Institutes at the University of California, San Francisco (UCSF). The users of the new equipment have a wide range of NIH-supported biomedical research projects and collectively boast many decades of experience with electron microscopy. Their interests include investigating genetic and acquired defects in the nuclear envelope, exploring the roles of different apolipoprotein E isoforms in neurodegenerative disease, defining the influence of G proteins on the development of cardiomyopathy, and studying the roles of triglyceride-synthesizing genes in the liver and intestine. Currently, the demand for electron microscopy far exceeds our capacity. The new microscope, with its digital imaging capabilities, will greatly accelerate the acquisition of ultrastructural images, making it possible to tackle many more research problems. Moreover, the new instrument, unlike older instruments, is much easier to use, opening the door to use by novices, in addition to professional electron microscopists. The new Electron Microscopy Core Facility will be run by Dr. Stephen G. Young. Day-to-day operations will be supervised by Ms. Jinny Wong, who has 30 years of continuous electron microscopy research experience. Dr. Robert Hamilton (UCSF professor of anatomy), a distinguished electron microscopist, will consult with the Core on technical and organizational issues. Gladstone is committed to the maintenance of the instruments and for providing salary support for the Core staff. The new electron microscope is absolutely critical to the ability of Gladstone investigators to train a new generation of biomedical investigators. Increasingly, the answers to biomedical research problems require a combination of biochemical and ultrastructural approaches. The new electron microscope will make it possible for us to teach our trainees a multidisciplinary approach to biomedical investigation.

DESCRIPTION (provided by applicant): The objective of this shared equipment grant is to obtain a JEOL JEM-1230 transmission electron microscope, a specimen rotation holder, a GATAN Ultrascan 1000 CCD camera, and a dedicated computer, for the community of biomedical investigators at the J. David Gladstone Institutes at the University of California, San Francisco (UCSF).新设备的用户拥有广泛的NIH支持生物医学研究项目，并且在电子显微镜上统一拥有数十年的经验。他们的兴趣包括研究核膜中的遗传和获得的缺陷，探索不同载脂蛋白E同工型在神经退行性疾病中的作用，定义了G蛋白对心肌病发展的影响，并研究了曲酯合成基因在Liver and Intestine中的作用。目前，对电子显微镜的需求远远超过了我们的能力。具有数字成像功能的新显微镜将极大地加速对超微结构图像的获取，从而有可能解决更多的研究问题。此外，与较旧的乐器不同，新仪器除了专业电子显微镜外，还可以更容易使用新手。新的电子显微镜核心设施将由Stephen G. Young博士运营。日常运营将由Jinny Wong女士进行监督，Jinny Wong女士拥有30年的连续电子显微镜研究经验。杰出的电子显微镜医生Robert Hamilton博士（UCSF解剖学教授）将就技术和组织问题的核心咨询。 Gladstone致力于维护乐器，并为核心人员提供薪水支持。新的电子显微镜对于Gladstone研究人员培训新一代生物医学研究人员的能力绝对至关重要。生物医学研究问题的答案越来越多，需要生化和超微结构方法的结合。新的电子显微镜将使我们有可能向学员教会生物医学研究的多学科方法。