GLYCOSYL COMPOSITION ANALYSIS OF MANDUCA SEXTA CHITINASE

烟草天蛾几丁质酶的糖基组成分析

• 批准号: 6653603
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653603
• 关键词: bacteria; biological products; biomedical resource; carbohydrates; spectrometry

The samples were analyzed for monosaccharide composition by preparing the trimethylsilyl derivatives of the methylglycosides followed by gas chromatography (GC) and combined gas chromatography/mass spectrometry (GC-MS) analysis. TMS methylglycosides were prepared from the samples by methanolysis in 3 M HCl followed by N-acetylation with pyridine and acetic anhydride. The samples were then treated with Tri-Sil. GC analysis of the TMS methylglycosides was done on an HP5890 GC using a DB1 fused silica capillary column. GC-MS analysis was performed using an HP5890 GC coupled to a 5970 MSD. Monosaccharides were identified by their retention times in comparison to standards, and the carbohydrate character of these was authenticated by their mass spectra.

通过以下方法分析样品的单糖组成： 制备甲基糖苷的三甲基甲硅烷基衍生物 然后进行气相色谱 (GC) 和合并气体分析 色谱/质谱 (GC-MS) 分析。 经颅磁刺激 甲基糖苷是通过在 3 M 中甲醇解从样品中制备的 HCl 然后用吡啶和乙酸酐进行 N-乙酰化。 这 然后用 Tri-Sil 处理样品。 TMS 的 GC 分析 甲基糖苷的分析是在 HP5890 GC 上使用 DB1 熔融石英进行的 毛细管柱。 使用 HP5890 GC 进行 GC-MS 分析 与 5970 MSD 耦合。 单糖的鉴定是通过它们的 与标准品相比的保留时间以及碳水化合物 这些的特征通过它们的质谱得到验证。