GLYCOSYL COMPOSITION ANALYSIS OF SACCHARIDE

糖的糖基组成分析

• 批准号: 6653596
• 负责人: ROBERTA K MERKLE
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653596
• 关键词: biological products; biomedical resource; carbohydrates; oral pharyngeal; spectrometry

Glycosyl-linkage analysis was performed by preparing permethylated alditiol acetates using the Hakomori procedure. The samples were dissolved in DMSO, DMSO anion was added, and the samples were stirred at room temperature for 2 h. An excess of methyliodide was added, and the samples were stirred again from 2 to 4 h at room temperature. Excess methyliodide was removed by evaporation, and the samples were purified using a C18 SepPak cartridge. The resulting samples were hydrolyzed in 2 M TFA, and the monosaccharides were reduced to their alditiols and N-acetylated using acidic anhydride in pyridine. The resulting acetates were then analyzed by GC-MS using a 30 mm SP2330 column from Supelco for the neutral sugars and using a DB1 column from J&W Scientific for the methylated amino sugars.

通过制备全甲基化的糖基连接分析 使用 Hakomori 程序生成醛糖醇乙酸酯。 样品是 溶解在DMSO中，加入DMSO阴离子，搅拌样品 在室温下放置2小时。加入过量的碘甲烷， 将样品在室温下再次搅拌2至4小时。 蒸发除去过量的碘甲烷，并将样品 使用 C18 SepPak 柱纯化。 所得样品为 在2M TFA中水解，单糖被还原为它们的 醛糖醇并用吡啶中的酸酐进行 N-乙酰化。 这 然后使用 30 mm SP2330 通过 GC-MS 分析所得乙酸盐 用于中性糖的 Supelco 色谱柱，并使用来自 J&W Scientific 用于甲基化氨基糖。