Analytical and Biophysical Research Core

分析和生物物理研究核心

• 批准号: 10611851
• 负责人: Joshua S Sharp
• 金额: $ 34.75万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611851
• 关键词: Address; Aerosols; Affinity; Analytical Chemistry; Binding; Bioinformatics; Biological; Biological Assay; Biomedical Research; Biophysics; Carbohydrates; Carbon; Centers of Research Excellence; Characteristics; Charge; Chemistry; Chromatography; Collaborations; Communities; Complex; Complex Mixtures; Computer software; Coupled; Data; Data Analyses; Dedications; Derivation procedure; Detection; Development; Diagnostic; Equipment; Experimental Designs; Fluorescence; Glycobiology; Glycoconjugates; Glycosaminoglycans; Glycosides; Growth; High Pressure Liquid Chromatography; Institution; Link; Mass Spectrum Analysis; Measures; Methods; Mississippi; Modernization; Modification; Natural Products; Nature; Neurosciences; Pathway Analysis; Pathway interactions; Phase; Pilot Projects; Polysaccharides; Porosity; Preparation; Protein-Carbohydrate Interaction; Proteome; Proteomics; Research; Research Personnel; Resolution; Resources; Role; Sampling; Science; Services; Spectrophotometry; Statistical Data Interpretation; Statistical Models; Structure; Structure-Activity Relationship; Support System; Surveys; System; Systems Biology; Techniques; Technology; Testing; Training; Universities; X-Ray Crystallography; absorption; biophysical analysis; biophysical chemistry; biophysical properties; biophysical techniques; chemical release; chemical resource; computational chemistry; data modeling; depolymerization; design; flexibility; instrumentation; interest; investigator training; metabolome; metabolomics; physical property; programs; protein metabolite; secondary metabolite; tandem mass spectrometry; tool

Project Summary/Abstract The University of Mississippi proposes to establish the Glycoscience Center of Research Excellence (GlyCORE), a Phase I Center of Biomedical Research Excellence. Glycoscience is an essential and rapidly emerging field of biomedical science, and the challenging nature of glycoscience research requires tools and expertise that are not commonly found in the biomedical research community. Moreover, the biomedical community is rapidly growing to appreciate the essential role of carbohydrates, with researchers outside of the self-identified “glycoscience” community finding themselves faced with addressing challenging questions in glycoscience as part of the course of their biomedical researchers. The focus of the Analytical and Biophysical Research Core is three-fold: to train investigators new to glycoscience in proper carbohydrate sample preparation and handling; to support hypothesis-driven research with targeted quantitative analyses from both enriched samples and complex mixtures; and to support discovery-based research to give investigators a system-level view of changes upon perturbation of targeted systems. Training of researchers is a key function of the Core, especially in Phase I when the institutional level of expertise in glycoscience is in its earliest stage of development. Core 1 will train researchers in carbohydrate and carbohydrate conjugate sample preparation techniques. Targeted analyses will be provided as a key technology for hypothesis-driven research, where an investigator wants to test for the presence and quantity of a given glycan or glycoside, structurally characterize an unknown molecule, or validate a key biological or chemical resource. Structural analyses of glycan modification, protein-carbohydrate interactions, binding affinity estimations, and other biophysical analyses will be supported through MS-, LC- and spectrophotometry-based technologies. Access to NMR instrumentation and expertise in spectral acquisition and interpretation will also be provided. Finally, Core 1 will offer “omics”-style discovery-based surveys of glycomes, proteomes, metabolomes, etc. in quantitative, semi-quantitative, and qualitative fashions, supporting systems glycobiology and hypothesis development for targeted assay design. Core 1 will interface closely with the Computational Chemistry and Bioinformatics Core to provide advanced statistical and pathway analyses for “omics” data. The services of Core 1 will use both existing University of Mississippi resources as well as a dedicated high resolution Thermo Q-Exactive Plus LC-MS/MS system and a Dionex Ultimate 3000 analytical UHPLC system. Due to the poor UV-absorption characteristics of most carbohydrates, fluorescence detection for tagged carbohydrates and charged aerosol detection of underivatized carbohydrates will also be available for sensitive detection and quantitation at high dynamic range. By focusing our Phase I Analytical and Biophysical Core on a small number of flexible technologies, we can support the diverse glycoscience interests of UM investigators with cutting-edge technology using a limited array of equipment and have room for growth as GlyCORE matures.

项目摘要/摘要 密西西比大学的提议建立了卓越研究中心 （Glycore），I阶段生物医学研究卓越中心。糖科学是必不可少的 生物医学科学的新兴领域以及糖科学研究的挑战性质需要工具和 在生物医学研究界没有常见的专业知识。而且，生物医学 社区正在迅速发展，以欣赏碳水合物的重要作用，研究人员在 自我识别的“糖科学”社区发现自己面临着解决挑战问题的 作为其生物医学研究人员过程的一部分，糖科学。分析和生物物理的重点 研究核心是三倍：在适当的碳水化合物样品中培训新的糖科学的研究者 准备和处理；通过有针对性的定量分析来支持假设驱动的研究 富集的样品和复杂的混合物；并支持基于发现的研究以为调查人员提供 对目标系统扰动的变化的系统级别的视图。研究人员的培训是关键 核心的功能，尤其是在第一阶段的核科学专业知识水平 最早的发展阶段。 Core 1将培训研究人员的碳水合物和碳水化合物结合物 样品制备技术。有针对性的分析将作为假设驱动的关键技术 研究人员想测试给定的聚糖或糖苷的存在和数量， 结构上表征未知分子，或验证关键的生物学或化学资源。结构 聚糖修饰，蛋白质 - 碳水化合物相互作用，结合亲和力估计和其他分析 生物物理分析将通过基于MS-，LC和基于分光光度计的技术支持。使用权 还将提供NMR仪器和光谱获取和解释的专业知识。最后， Core 1将提供“ OMICS”风格的基于发现的甘氨酸，蛋白质组，代谢组等的调查。 定量，半定量和定性时尚，支持系统糖生物学和假设 针对目标测定设计的开发。核心1将与计算化学和 生物信息学核心为“ OMICS”数据提供高级统计和途径分析。服务 Core 1将同时使用现有的密西西比大学资源以及专用的高分辨率Thermo Q-Extractive Plus LC-MS/MS系统和Dionex Ultimate 3000分析UHPLC系统。由于贫穷 大多数碳水合物的紫外吸收特征，标记的碳水合物的荧光检测和 还可以使用带电的气溶胶检测碳化物碳水化合物的敏感检测和 高动态范围的定量。通过将我们的I期分析和生物物理核心集中在一个小的 灵活的技术数量，我们可以支持UM调查人员的潜水员聚糖兴趣 尖端技术使用有限的设备，并随着聚糖的成熟而有生长空间。