HITACHI S-3000N VP SCANNING ELECTRON MICROSCOPE

日立 S-3000N VP 扫描电子显微镜

• 批准号: 6288114
• 负责人: JOHN J EPPIG
• 金额: $ 28.73万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6288114
• 关键词: biomedical equipment purchase; scanning electron microscopy

The generation and characterization of mouse strains harboring genetically predetermined pathobiological syndromes is critical to biomedical research, and researchers at The Jackson Laboratory (TJL) are at the forefront of this endeavor. Scanning electron microscopy (SEM) represents a powerful method for imaging, documenting and quantifying in situ the morphological changes in cells and tissues associated with normal and induced genetic variation. Over 80% of TJL research staff rely upon SEM for such characterization. The Biological Imaging Service currently operates a twenty-year old scanning electron microscope. Due to age, its technology is outdated, its application is inflexible, resolution is limited and instrument performance is increasingly unreliable. Higher levels of resolution, variable pressure operation, multiple specimen holder, viewing chamber, flexible stage movement and digital image capture are considered state-of-the-art features; all are available on the Hitachi S-3000N Variable Pressure Scanning Electron Microscope. This unit is fitted with a cooling stage for optimal preparation of water-containing specimens and its ability to operate variable pressure mode permits image collection of uncoated specimens introducing time and cost savings. Physical constraints and finite stage movement of the current SEM limit the number of samples that can be loaded and analyzed; digital data collection is not possible. The Hitachi-S3000N offers users multi-specimen capabilities and networked digital image acquisition, elemental analysis and post processing management. The Hitachi S-3000N Variable Pressure Scanning Electron Microscope would fulfill the urgent and unmet SEM needs of TJL's staff. Introducing a stable instrument that enhances the scope, quality and speed of data collection will further advance 28 NIH-funded research programs of 15 investigators on campus and support the development of new NIH applications.

具有基因预定的病理生物学综合征的小鼠品系的产生和表征对于生物医学研究至关重要，杰克逊实验室 (TJL) 的研究人员处于这一努力的最前沿。扫描电子显微镜 (SEM) 是一种强大的方法，可对与正常和诱导遗传变异相关的细胞和组织的形态变化进行原位成像、记录和量化。超过 80% 的 TJL 研究人员依靠 SEM 来进行此类表征。生物成像服务中心目前使用一台已有二十年历史的扫描电子显微镜。由于年代久远，其技术陈旧，应用不灵活，分辨率有限，仪器性能越来越不可靠。更高水平的分辨率、可变压力操作、多个样品架、观察室、灵活的载物台移动和数字图像捕获被认为是最先进的功能；所有这些都可以在 Hitachi S-3000N 变压扫描电子显微镜上实现。该装置配有冷却台，可实现含水样品的最佳制备，并且其操作可变压力模式的能力允许对未涂层样品进行图像采集，从而节省时间和成本。当前 SEM 的物理约束和有限的载物台移动限制了可加载和分析的样品数量；数字数据收集是不可能的。 Hitachi-S3000N 为用户提供多样本功能以及网络数字图像采集、元素分析和后处理管理。 Hitachi S-3000N 变压扫描电子显微镜将满足 TJL 员工迫切且未满足的 SEM 需求。引入可提高数据收集范围、质量和速度的稳定仪器将进一步推进 NIH 资助的 28 项研究项目，该项目由校园内 15 名研究人员负责，并支持 NIH 新应用的开发。