SCANNING ELECTRON MICROSCOPY SYSTEM

扫描电子显微镜系统

• 批准号: 6287981
• 负责人: John J Godleski
• 金额: $ 31.58万
• 依托单位: HARVARD SCHOOL OF PUBLIC HEALTH
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6287981
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; scanning electron microscopy

Goal: This revised application seeks funds to replace an existing scanning electron microscope (SEM with a state-of-the-art low vacuum scanning microscope/x-ray analysis system, the JSM-5600LV with a fully quantitative and advanced imaging X-Ray microanalysis system. Problem: The core of our present system is an Amray 1000a with a Kevex mu x 7000 Energy Dispersive X-ray Spectroscopy (EDS) system. Our SEM was manufactured in 1975. The Kevex mu x 7000 EDS is also of that vintage.While the instrument has provided many years of service, the system is out-dated and inadequate for current applications. In addition, parts are difficult if not impossible to obtain, so that a number of standard functions on our system are inoperable. Furthermore, the SEM does not have the capacity to produce or store digital information. The user group for this instrument has grown and requires significantly improved capabilities available on modem scanning electron microscopes, specifically higher magnification, digital images, variable energy ranges, as well as higher maximum energy, variable vacuum pressures, and x-ray analysis of individual environmental particles, including automated analysis for cell and metal studies. Significance: The instrumentation will serve an established and productive group of investigators performing NIH-sponsored research. Current use includes genetic/morphological comparisons, cell surface relationship among cell types following metal exposure, analysis of environmentally relevant particle composition, size and surface area. Scanning Electron Microscopy has become an indispensable analytical tool required to complete several of our funded studies. The instrumentation is part of a dedicated Bioimaging Core facility whose personnel, service and maintenance costs are supported by an NIEHS center grant and several program projects. There is growing demand for the use of the facility. Institutional support is demonstrated by past support of the facility, completed renovations, commitments for ongoing service contracts, and letters of commitment for future support.

目标：本修订后的申请寻求资金替换现有的扫描电子显微镜（配备最先进的低真空扫描显微镜/X 射线分析系统的 SEM、配备完全定量和先进成像 X 射线的 JSM-5600LV）问题：我们现有系统的核心是配备 Kevex mu x 7000 能量色散 X 射线光谱 (EDS) 系统的 Amray 1000a。 1975 年。Kevex mu x 7000 EDS 也是那个年份的。虽然该仪器已经使用多年，但该系统已经过时且不足以满足当前的应用。此外，零件即使不是不可能获得也很困难。我们系统上的许多标准功能无法运行，而且 SEM 无法生成或存储数字信息。该仪器的用户群不断增长，需要显着改进现代扫描电子显微镜的功能，特别是更高的功能。放大倍率、数字图像、可变能量范围以及更高的最大能量、可变真空压力和单个环境颗粒的 X 射线分析，包括细胞和金属研究的自动分析。意义：该仪器将为执行 NIH 资助的研究的成熟且富有成效的研究人员群体提供服务。目前的用途包括遗传/形态比较、金属暴露后细胞类型之间的细胞表面关系、环境相关颗粒成分、大小和表面积的分析。扫描电子显微镜已成为完成我们资助的几项研究所需的不可或缺的分析工具。该仪器是专用生物成像核心设施的一部分，其人员、服务和维护成本由 NIEHS 中心拨款和多个计划项目支持。使用该设施的需求不断增长。机构支持通过设施过去的支持、已完成的翻修、持续服务合同的承诺以及未来支持的承诺书来证明。