IMAGING TRANSMISSION ELECTRON MICROSCOPE

成像透射电子显微镜

• 批准号: 6288104
• 负责人: VIRGINIA M PICKEL
• 金额: $ 40.55万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6288104
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; brain imaging /visualization /scanning; protein localization; transmission electron microscopy

High resolution electron microscopic immunolabeling of recently cloned proteins now permits clear identification of sites mediating brain functions that are affected in neurological and neurophychiatric diseases. This unique capability, together with a greater demand for quantitative ultrastructural data, has produced an unprecedented need for the use of a modern transmission electron microscope (TEM) by the faculty within the Departments of Neurology and Neuroscience and Pharmacology at Weill Medical College of Cornell University (WMC-CU). Original studies from this group have provided fundamental information on the subcellular distribution of neurotransmitter transporters and receptors using primarily a 1973 model of a Philips 201 TEM, which has become obsolete and unable to cost-effectively meet the needs of the investigators. This proposal requests funds to purchase a new TEM (Tecnai 12 BioTwin) with a digital camera and capacity for on-line imaging and quantitative analysis. The availability of this technologically advanced system will assure the completion of major biomedical research projects that are funded from several different branches of the NIH. These funds cannot be obtained from the research grants of any one investigator, and are not available except through the NIH Shared Instrument Program. The funded projects of the six principal investigators (P.I.'s) who comprise the major users group in this proposal rely exclusively on a TEM for data analysis. These studies require the use of a TEM for quantitatively determining the normal subcellular distribution and drug- or lesion-induced changes in the targeting of opioid receptors and other functional proteins within the rat central nervous system (CNS). The projects of six other investigators who comprise the minor users group require more limited, but crucial, on-line TEM analysis of CNS antigens, particularly in genetic animal models of neurodegenerative diseases and drug addiction. Together, the results from these studies will have major implications for understanding the control of pain, autonomic functions, motivated behaviors, and memory in humans. To assure the successful long-term use of the new TEM system, the daily operation of the instrument will be managed by an internal advisory committee of all major users, and maintained by an experienced staff with the full support of the Medical College.

最近克隆的蛋白质的高分辨率电子显微镜免疫标记现在可以清楚地识别介导受神经系统和神经精神疾病影响的大脑功能的位点。这种独特的功能，加上对定量超微结构数据的更大需求，使得康奈尔大学威尔医学院神经病学、神经科学和药理学系的教师对使用现代透射电子显微镜 (TEM) 产生了前所未有的需求（WMC-CU）。该小组的原始研究主要使用 1973 年的 Philips 201 TEM 模型，提供了有关神经递质转运蛋白和受体的亚细胞分布的基本信息，该模型已经过时，无法经济高效地满足研究人员的需求。该提案要求资金购买一台新的 TEM (Tecnai 12 BioTwin)，配备数码相机和在线成像和定量分析能力。这种技术先进的系统的可用性将确保完成由美国国立卫生研究院几个不同部门资助的重大生物医学研究项目。这些资金不能从任何一位研究者的研究资助中获得，并且只能通过 NIH 共享仪器计划获得。构成本提案中主要用户组的六名主要研究人员 (P.I.) 的资助项目完全依赖 TEM 进行数据分析。这些研究需要使用 TEM 来定量确定大鼠中枢神经系统 (CNS) 内阿片受体和其他功能蛋白靶向的正常亚细胞分布以及药物或病变引起的变化。构成未成年用户群体的其他六名研究人员的项目需要对 CNS 抗原进行更有限但至关重要的在线 TEM 分析，特别是在神经退行性疾病和药物成瘾的遗传动物模型中。总之，这些研究的结果将对理解人类疼痛的控制、自主功能、动机行为和记忆产生重大影响。为了确保新TEM系统的长期成功使用，仪器的日常运行将由所有主要用户组成的内部咨询委员会进行管理，并由经验丰富的工作人员在医学院的全力支持下进行维护。