Acquisition of a Cryogenic Field Emission EM

获取低温场发射电子显微镜

• 批准号: 6732576
• 负责人: DAVID H THOMPSON
• 金额: $ 50万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6732576
• 关键词: biomedical equipment purchase; cryoelectron microscopy; scanning electron microscopy

DESCRIPTION (provided by applicant): The need to visualize fine structural details in samples that are as close to the natural state as possible is increasingly important in modern biology and related biomedical research. This proposal requests funds for the purchase of a Hitachi S-4800 Field Emission Scanning Electron Microscope equipped with a Gatan cryotransfer system. This instrument will provide capabilities necessary for imaging biological samples and relatively soft materials having features on the nanometer length scale under low accelerating voltages and, where applicable, fully hydrated condition using modern cryogenic technology. Virtually all of the investigators included in this proposal intend to use this instrument on low atomic number, non-conductive samples, with the majority being hydrated samples that require a cryogenic preparation and viewing to preservation of structural detail. Some of the research that will be impacted by acquisition of this instrument are drug and gene delivery, biomolecular & bioelectronic signaling, molecular based sensing systems and other important new technologies in biomedical research.

描述（由申请人提供）：在现代生物学和相关生物医学研究中，对尽可能接近自然状态的样品中精细结构细节进行可视化的需求变得越来越重要。该提案请求资金购买配备 Gatan 低温转移系统的日立 S-4800 场发射扫描电子显微镜。该仪器将提供在低加速电压和（如果适用）完全水合条件下使用现代低温技术对生物样品和具有纳米长度尺度特征的相对软材料进行成像所需的功能。事实上，该提案中包括的所有研究人员都打算在低原子序数、非导电样品上使用该仪器，其中大多数是需要低温制备和观察以保存结构细节的水合样品。收购该仪器将影响的一些研究包括药物和基因传递、生物分子和生物电子信号、分子传感系统以及生物医学研究中的其他重要新技术。