CORE--MASS SPECTROMETRY LABORATORY

核心--质谱实验室

• 批准号: 6630907
• 负责人: VERNON R YOUNG
• 金额: $ 10.37万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6630907
• 关键词: biomedical facility; mass spectrometry; nutrition; nutrition related tag; radiotracer

Mass spectrometric techniques, particularly those requiring the measurement of stable (heavy) nuclide enrichment in biological matrices have become essential tools in the study of nutrition and metabolism under various pathophysiological conditions. The nutrition/metabolic research program within the CNRU has given particular impetus to this area over the past years and it continues to be directed towards a better definition and characterization of the changes in nutrient, fuel and substrate metabolism that accompany various pathophysiological states. A part-time manager will support this core facility together with a well trained full-time technician. The principal functions of the mass spectrometry facility are four: 1. To provide timely and accurate determinations of stable isotope enrichments of tracers and their derivatives in bodily fluids, tissues, and expired air. 2. To assist in development of new methods for isotope analyses of compounds in blood tissues, and urine, as required to meet the needs of the investigators who are participating in the Center Grant research program. 3. To provide quality control for all analysis conducted in support of the research studies that involve use of stale nuclides as tracers. 4. To serve a training function and provide potential new investigators with an opportunity to learn about the application of stable isotope-tracer techniques in metabolic/nutritional research.

质谱技术，尤其是那些需要测量稳定（重）核素富集在生物基质中的技术已成为各种病理生理条件下营养和代谢研究的基本工具。 在过去几年中，CNRU中的营养/代谢研究计划已为该领域提供了特殊的动力，并且它继续致力于更好地定义和表征伴随各种病理生理状态的营养，燃料和底物代谢的变化。 兼职经理将与训练有素的专职技术人员一起支持此核心设施。质谱设施的主要功能是四：1。在体液，组织和过期的空气中，及时，准确地确定迹线及其衍生物的稳定同位素富集及其衍生物。 2。协助开发血液组织中化合物和尿液中同位素分析的新方法，以满足参加中心赠款研究计划的研究人员的需求。 3。为所有分析提供质量控制，以支持涉及使用陈旧核素作为示踪剂的研究。 4。服务培训功能并为潜在的新研究人员提供机会，以了解稳定的同位素跟踪技术在代谢/营养研究中的应用。