Biomolecular Analysis Core D

生物分子分析核心 D

• 批准号: 7116101
• 负责人: JOHN W TURK
• 金额: $ 12.64万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116101
• 关键词: biomedical facility; clinical research; gas chromatography; mass spectrometry; matrix assisted laser desorption ionization; method development; nutrition

The Biomolecular Analysis Core increases efficiency and cost effectiveness by performing mass spectrometric and certain other analyses for CNRU investigators. The Core provides state-of-the-art mass spectrometry to determine structures and to quantify nutrients and related substances in biological specimens. The Core provides centralized, standardized analytical procedures that permit study of molecular mechanisms for nutrient-related physiologic and pathophysiologic events. Specific objectives of the Biomolecular Analysis Core are: To provide training to students and fellows in principles of mass spectrometry (MS) and use of MS systems, e.g., gas chromatography (GC)/MS, isotope ratio (IR)/MS, electrospray ionization (ESI)/tandem MS, and matrix assisted laser desorption ionization (MALDI)/time of flight/ (TOF)/MS in analysis of nutrition-related biomolecules. To develop new MS methods for structural identification and quantitation of molecules involved in nutrition-related physiologic and pathophysiologic events. To perform service-related MS analyses, e.g. quantitation of target analytes and obtaining spectra for structural identification. To assist CNRU investigators in developing MS assays. To provide consultation in interpreting MS data. To develop and disseminate new approaches in biomedical MS applicable to nutrition research. To provide and maintain functional MS systems for use in nutrition research. To perform collaborative nutrition research studies involving Biomolecular Analysis Core staff. To reduce nutrition research-related costs by providing centralized MS services and consultation at a fraction of the cost for commercial MS services or of maintaining instruments in individual investigators' laboratories.

生物分子分析核心通过执行质量提高效率和成本效益 CNRU研究人员的光谱和某些其他分析。核心提供最先进的质量 光谱法确定结构并量化生物学的养分和相关物质 标本。核心提供了集中的，标准化的分析程序，可以研究 营养相关生理和病理生理事件的分子机制。 生物分子分析核心的特定目标是： 为学生和研究员提供质谱原理（MS）的培训和MS的使用 系统，例如气相色谱（GC）/MS，同位素比（IR）/MS，电喷雾电离 （ESI）/串联MS和矩阵辅助激光解吸电离（MALDI）/飞行时间/（TOF）/MS in 与营养相关的生物分子的分析。 开发新的MS方法，用于鉴定和定量涉及的分子 与营养有关的生理和病理生理事件。 要执行与服务相关的MS分析，例如定量目标分析物并获得光谱 用于结构识别。 帮助CNRU研究人员开发MS分析。 在解释MS数据时提供咨询。 开发和传播适用于营养研究的生物医学MS中的新方法。 提供和维护功能性MS系统，用于营养研究。 进行涉及生物分子分析核心人员的协作营养研究。 通过提供集中的MS服务和咨询来降低与营养研究相关的成本 商业MS服务或个人维护工具的一小部分 调查人员的实验室。