CELLULAR DEFENSE RESPONSE IN AN INSECT SYSTEM

昆虫系统中的细胞防御反应

• 批准号: 6555588
• 负责人: Michael Strand
• 金额: $ 23.63万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6555588
• 关键词: Lepidoptera; antisense nucleic acid; blood cells; cell adhesion; cell adhesion molecules; cell capsule; cell population study; cellular immunity; cytotoxicity; flow cytometry; gene expression; host organism interaction; in situ hybridization; integrins; molecular cloning; monoclonal antibody; nuclear magnetic resonance spectroscopy; parasitism; plasma cells; polymerase chain reaction; protein binding; protein structure function; tissue /cell culture

DESCRIPTION (Adapted from the Applicant's Application): This is a 5-year competitive renewal of a proposal entitled 'Cellular defense response in an insect system.' In the previous proposal the PI has developed an elegant system to investigate the encapsulation of a foreign object by hemocytes from larvae of the moth, Pseudoplusia includens. These studies are significant because encapsulation constitutes a fundamental component of the insect immune response. In this competing renewal, the PI will continue his investigation of the two cell types, plasmatocytes and granular cells that participate in this response. He will also extend previous work on a plasmatocyte spreading peptide (PSP1), which induces plasmatocytes to undergo a change in adhesive state and to release specific effector molecules. The present application has 4 specific aims: 1. To characterize structural features of PSP1 essential for activity; 2, to identify a PSP1 receptor, 3, to identify molecules that mediate plasmatocyte adhesion, and 4, to examine characteristics of plasmatocyte-induced cytotoxicity. The availability of large numbers of hemocytes from Pseudoplusia includens, and the specific techniques and approaches developed in the PI's laboratory are strong arguments in favor of this experimental system, which will provide important comparative insights into immunity functions in arthropod vectors.

描述（改编自申请人的申请）：这是一个为期 5 年的项目 题为“细胞防御反应”的提案的竞争性更新 昆虫系统。在之前的提案中，PI 开发了一个优雅的系统 研究幼虫血细胞对异物的封装 蛾类，Pseudoplusia includens。这些研究意义重大，因为 封装是昆虫免疫的基本组成部分 回复。在这次竞争更新中，PI 将继续调查 参与此过程的两种细胞类型：浆细胞和颗粒细胞 回复。他还将扩展之前关于浆细胞传播肽的工作 (PSP1)，诱导浆细胞发生粘附状态的变化并 释放特定的效应分子。本申请有4个具体 目标： 1. 表征 PSP1 活性所必需的结构特征； 2、 识别 PSP1 受体，3，识别介导浆细胞的分子 粘附，4，检查浆细胞诱导的特性 细胞毒性。可获得大量来自伪斑纹藻的血细胞 包括，以及 PI 中开发的具体技术和方法 实验室有强有力的论据支持这个实验系统， 将为免疫功能提供重要的比较见解 节肢动物载体。