ENZYMOLOGICAL ASPECTS OF NEURAL FUNCTIONS

神经功能的酶学方面

• 批准号: 6432872
• 负责人: ROBERT W ALBERS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6432872
• 关键词: active sites; calmodulin dependent protein kinase; cations; chemical kinetics; enzyme activity; enzyme mechanism; enzyme structure; ion transport; mass spectrometry; neurochemistry; osmosis; phosphorylation; posttranslational modifications; protein structure function; sodium potassium exchanging ATPase; solvents; water

The principal goal of this project is to elucidate function of the sodium pump, in particular we are interested in the transient kinetics of the sodium pump. A collaboration with J. Froehlich of NIA is directed at determining the extent to which oligomeric interactions of the sodium pump contribute to the efficiency of cation transport. We have obtained evidence, by a variety of techniques, that "out-of-phase" interactions of two or more alpha subunits occur in the native state of this and other P-type cation pumps.

该项目的主要目标是阐明钠泵的功能，特别是我们对钠泵的瞬时动力学感兴趣。 与NIA的J. Froehlich进行的合作旨在确定钠泵的低聚相互作用在多大程度上有助于阳离子运输的效率。 我们通过多种技术获得了证据，表明两个或多个α亚基的“相外”相互作用发生在该和其他P型阳离子泵的天然状态。