ENZYMOLOGICAL ASPECTS OF NEURAL FUNCTIONS

神经功能的酶学方面

• 批准号: 2579498
• 负责人: R W ALBERS
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2579498
• 关键词: active sites; calmodulin dependent protein kinase; cations; chemical kinetics; enzyme activity; enzyme mechanism; enzyme structure; ion transport; mass spectrometry; neurochemistry; osmosis; phosphorylation; posttranslational modifications; protein structure function; sodium potassium exchanging ATPase; solvents; water

There are currently three studies within this project: 1) Transient kinetics of the sodium pump: Investigations into the cause of the biphasic characteristics of catalytic site phosphorylation and dephosphorylation, in collaborations with Froehlich, Fendler and Taniguchi. 2) Regulatory modifications of the sodium pump: Current studies are directed toward testing the hypothesis that the great sensitivity of P-type cation pumps to certain solvents is an "osmotic stress" effect on the accessibility of water to certain sites in these pump molecules. 3) Regulatory modifications of calmodulin-dependent kinase II: Studies of the functional consequences of the autophosphorylation states of calmodulin-dependent kinase II, in collaboration with A. Dosemeci.

该项目目前目前有三项研究： 1）钠泵的瞬时动力学：对原因的研究 催化位点磷酸化的双相特征和 与Froehlich，Fendler和 Taniguchi。 2）钠泵的调节修改：当前的研究是 致力于检验以下假设 P型阳离子泵到某些溶剂是一种“渗透应力”效应 关于这些泵分子中某些位点的水的可及性。 3）钙调蛋白依赖性激酶II的调节修饰：研究 自磷酸化状态的功能后果 康复蛋白依赖性激酶II，与A. dosemeci合作。