DIGITAL MICROSCOPY/IMAGE ANALYSIS SYSTEM

数字显微镜/图像分析系统

• 批准号: 6051638
• 负责人: MARTIN DYM
• 金额: $ 20.28万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051638
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; image processing; light microscopy

We are requesting funds to purchase an integrated state-of-the-art digital light microscopic image analysis system to serve a number of faculty members and their research associates in the Department of Cell Biology at Georgetown University. The light microscopes in our department are older units with no capacity to integrate with the computer or with the modern image analysis software that is now available. All of the Major Users on this application only have access to photomicroscopic equipment that is outdated. The objectives that we are using are not the best ones available, thus, in many instances the image quality suffers. None of the Major Users has a high-resolution digital camera that could be attached to a microscope and integrated with a computer. Similarly, the available image analysis system is now quite limited and unable to meet the requirements of our Major Users. We need a state-of-the-art image analysis system since each of us carries out quantitative analysis on histological slides, both for immunolocalization, in situ hybridization, and for morphometry. The image analysis software will greatly facilitate our data analysis and maximize the validity of the data. The new microscope will be equipped with bright-field, dark-field, phase, differential interference contrast, and fluorescence optics. As part of the system, we hope to purchase a dye sublimation color printer. The equipment that we wish to buy will also enable us to bypass the traditional darkroom and traditional film. We will demonstrate in this grant application that it will be cost effective to purchase two digital cameras, one specifically for color bright-field and the second, a cooled camera, for low light fluorescence. The Department of Cell Biology recently purchased a computer, a flat bed scanner, a 35-mm slide scanner, and a film recorder to produce the highest quality 35-mm slides directly from software programs such as PowerPoint or CorelDraw, and thus we have initiated the establishment of a departmental shared "Digital Darkroom" as part of our Morphology Core. The light microscopic image analysis system with the digital cameras and printer that we are requesting in this grant application will become an integral part of the Morphology Core's "Digital Darkroom".

我们要求资金购买综合的最先进的数字光显微图像分析系统，以服务于乔治敦大学细胞生物学系的许多教职员工及其研究伙伴。我们部门的光显微镜是较旧的单元，没有能力与计算机或现已可用的现代图像分析软件集成。该应用程序上的所有主要用户都只能访问过时的显微镜设备。我们使用的目标不是可用的最佳目标，因此，在许多情况下，图像质量受到了影响。没有一个主要用户都有高分辨率的数码相机，该数码相机可以连接到显微镜并与计算机集成。 同样，可用的图像分析系统现在非常有限，无法满足我们主要用户的要求。我们需要一个最先进的图像分析系统，因为我们每个人都对组织学载玻片进行定量分析，均用于免疫定位，原位杂交和形态计量学。图像分析软件将极大地促进我们的数据分析并最大程度地提高数据的有效性。新的显微镜将配备明亮场，深色场，相位，差异干扰对比度和荧光光学元件。作为系统的一部分，我们希望购买染料升华颜色打印机。我们希望购买的设备还将使我们能够绕过传统的暗室和传统电影。我们将在此赠款应用程序中证明，购买两个数码相机将是具有成本效益的，一个专门用于颜色明亮场，第二个是冷却相机，用于低光荧光。细胞生物学系最近购买了一台计算机，平板扫描仪，35毫米幻灯片扫描仪和胶卷录像机，可直接从PowerPoint或Coreldraw等软件程序中生产最高质量的35毫米滑梯，因此我们启动了作为我们形态学核心的一部分，建立了一个部门共享的“数字暗室”。我们在此赠款应用程序中要求使用的数码相机和打印机的光显微图像分析系统将成为形态核心“数字暗室”的组成部分。