LONG TERM CULTURE OF SPERMATOGONIAL STEM CELLS

精原干细胞的长期培养

• 批准号: 6181741
• 负责人: MARTIN DYM
• 金额: $ 22.12万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6181741
• 关键词: basement membrane; cell line; growth factor; laboratory mouse; laboratory rat; male; sperm; tissue /organ preservation

DESCRIPTION: This application will try to achieve the long term culture of type A spermatogonia. Three specific aims are proposed: 1) to determine the basement membrane components that are optimal for allowing long-term (longer than 1 week) survival of type A spermatogonia in culture; 2) to test the hypothesis that there is synergism between growth factors and basement membrane components in promoting long-term survival of spermatogonia; and 3) to develop immortalized cell lines of type A spermatogonia that will propagate in culture without losing their original properties.

描述：此应用程序将尝试实现长期文化 键入A精子。 提出了三个具体目标：1）确定 地下室膜成分，最适合长期允许 （超过1周）培养中A型精子的生存； 2）测试 假设生长因子与基底之间存在协同作用 促进精子生存的膜成分； 3） 开发A型A型的永生细胞系，将 在文化中传播而不会失去其原始特性。