Measurement of Cells In Vivo: IVIS Light Imaging System

体内细胞测量：IVIS 光成像系统

• 批准号: 6580062
• 负责人: THOMAS J ROSOL
• 金额: $ 17万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580062
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; cell sorting; cells; image processing; whole body imaging /scanning

DESCRIPTION (provided by applicant): An IVIS Imaging System made by Xenogen Corporation is requested in this Shared Instrument Grant application. The IVIS Imaging System uses a highly efficient CCD camera (in a light-free imaging chamber) and Livinglmage. Software to rapidly capture light emitted from luciferase to image human or animal cells, bacteria, viruses, or genes that are linked to luciferase expression. The instrument will allow fourteen NIH-funded investigators to complete and significantly improve their studies on tumor growth, pathogenesis of infectious disease, and gene expression in vivo using animal models of human disease. Importantly, the IVIS Imaging System will enable the investigators to complete longitudinal studies in vivo without sacrifice of experimental animals. The Imaging System can detect as few as 200 cells in the subcutis and can readily image luciferase expression by cells in lungs, lymph nodes, and bones of anesthetized mice (up to 10 mice at one time). Translational studies on human cancer in mice are usually limited by the ability to measure or image tumors in vivo. The IVIS Imaging System will permit accurate measurement of viable tumor cells in subcutaneous and visceral neoplasms, response to therapy, and greatly shorten the time and animals numbers needed to investigate the pathogenesis and treatment of human cancer in vivo. In addition, the Imaging System will allow investigators to monitor gene expression and pathogenic organisms in vivo. This technology will significantly improve the ability of the investigators to complete currently funded research projects and extend the capabilities of the investigators to propose new translational research projects that require in vivo imaging in mice or other small animals. There is no similar instrument at The Ohio State University, and only one IVIS Imaging System exists in Ohio (at Procter & Gamble Co., Cincinnati). The instrument will be housed in a barrier animal facility (Sisson Hall) in the College of Veterinary Medicine under the supervision of a Research Scientist Supervisor (that specializes in imaging) and a Laboratory Animal Veterinarian. The principal investigator has received training in use of the IVIS Imaging System in the laboratory of Chris Contag, PhD at Stanford University (developer of the IVIS System and founder of Xenogen Corp.). Users will be trained by the Principal Investigator and the supporting Research Scientist and Laboratory Animal Veterinarian. The software will operate in a Windows environment on a networked computer that will allow the investigators to access the data from distant sites. An hourly user fee will provide funds for a service contract and continuing education of the supervisor. Billing will be performed by the current system of the Imaging Services in the Department of Veterinary Biosciences. Space and utilities for the instrument plus salary and benefits for the supervisor will be provided by the Department of Veterinary Biosciences and the College of Veterinary Medicine.

描述（由申请人提供）： 本共享的工具赠款应用程序要求Xenogen Corporation制造的IVIS成像系统。 IVIS成像系统使用高效的CCD摄像头（在无光成像室中）和LivingLmage。软件可以快速捕获从荧光素酶发出的光，从而成像与荧光素酶表达相关的人类或动物细胞，细菌，病毒或基因。该仪器将允许14名NIH资助的研究人员使用人类疾病动物模型在体内完成并显着改善其对肿瘤生长，传染病发病机理以及体内基因表达的研究。重要的是，IVIS成像系统将使研究人员能够在体内完成纵向研究，而无需牺牲实验动物。成像系统可以检测到亚布丁中的最低200个细胞，并且可以轻易地通过肺，淋巴结和麻醉小鼠的骨骼中的细胞来表达荧光素酶的表达（一次最多10只小鼠）。小鼠中人类癌症的翻译研究通常受体内测量或图像肿瘤的能力的限制。 IVIS成像系统将允许在皮下和内脏肿瘤中准确测量可行的肿瘤细胞，对治疗的反应，并大大缩短了研究体内人类癌症的发病机理和治疗所需的时间和动物数量。此外，成像系统将允许研究人员在体内监测基因表达和致病生物。这项技术将显着提高研究人员完成当前资助的研究项目的能力，并扩展研究人员提出新的翻译研究项目的能力，这些研究需要在小鼠或其他小动物中进行体内成像。俄亥俄州立大学没有类似的工具，俄亥俄州只有一个IVIS成像系统（在辛辛那提Procter＆Gamble Co.）。该乐器将在研究科学家主管的监督下（专门从事成像）和实验室动物兽医的监督，将其安置在兽医学院的障碍动物设施（Sisson Hall）中。首席研究人员已接受了斯坦福大学（IVIS系统的开发商和Xenogen Corp.的创始人）的Chris Contag实验室中IVIS成像系统使用的培训。用户将由首席研究员和支持研究科学家和实验室动物兽医培训。该软件将在网络计算机上的Windows环境中运行，该计算机将允许研究人员从遥远的站点访问数据。每小时的用户费用将为主管的服务合同提供资金。计费将由兽医生物科学系的当前成像服务系统进行。仪器生物科学系和兽医学院将提供该工具的空间和公用事业，以及主管的薪水和福利。