Comparative Pathology Core

比较病理学核心

基本信息

批准号：
9091440
负责人：
THOMAS J ROSOL
金额：
$ 18.55万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-15 至 2018-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9091440
关键词：
Antigens Authorship Autopsy Bioinformatics Biopsy Biostatistics Core Cells Collaborations Comparative Pathology Computer software Computers Confocal Microscopy Consensus Data Data Analyses Data Collection Development Doctor of Philosophy Ensure Epithelial-Stromal Communication Equipment Evaluation Evolution Experimental Designs Faculty Freezing Genetic Models Goals Grant Head Health Services Research Histology Histopathology Human Human Pathology Human Resources Image Imaging Techniques Immunohistochemistry In Situ In Situ Hybridization Instruction Laboratories Laboratory mice Leadership Lesion Link Malignant Neoplasms Mammary Neoplasms Mammary gland Manuscripts Medical Medical Oncologist Methods MicroRNAs Microscope Microscopic Microscopy Molecular Molecular Target Monitor Mus Ohio Oncologist Operative Surgical Procedures Output Paraffin Tissue Pathogenesis Pathologist Pathology Pathology Report Phase Phenotype Photography Physiological Positioning Attribute Postdoctoral Fellow Procedures Proteins Proteome Protocols documentation Quality Control Reporting Research Research Project Grants Resolution Resource Sharing Resources Sampling Services Slide Specialist Staining method Stains Supervision System Time Tissue Banks Tissue Microarray Tissue Procurements Tissue Sample Tissues Translations Ultrasonography Universities Woman Work Writing base design digital fluorescence imaging fluorescence microscope genetic analysis high standard histological stains human tissue in vivo interest laser capture microdissection malignant breast neoplasm meetings member molecular pathology mouse model outcome forecast programs protocol development research study statistics symposium tissue processing transcriptome tumor tumor microenvironment tumor progression web site

项目摘要

PROJECT SUMMARY (See instructions): The Comparative Pathology Core A will provide centralized pathology support for the three scientific projects and represents an evolution from the Mouse Pathobiology Core from the initial grant cycle. A major goal of the Comparative Pathology Core will be to provide expertise in mouse and human pathology with up-to-date proficiency in mammary gland and breast cancer pathology. In addition, a DVM, PhD postdoc in mouse pathology will perform the mouse necropsies (autopsies), cutting and processing of tissues, and evaluation of histopathology and special stains under the supervision of faculty pathologists. This will ensure excellence and continuity of service for the research projects. The Core will have 6 personnel that include three pathologists (one medical and two veterinary), medical oncologist (breast cancer specialist), a pathology fellow, and part-time histology technician. The Comparative Pathology Core is essential to ensure integration of the specific aims and experiments in relation to translation of the mouse genetic models to human breast cancer. The three pathologists and oncologist have a long track record of working together on collaborative projects, and therefore are well positioned to provide support for Core A. In addition, all three pathologists and the oncologist have collaborated with Drs. Ostrowski and Leone leading to shared authorship on manuscripts with high impact on mammary cancer microenvironment and its relationship to mammary cancer progression (1, 2). Facilities will include four necropsy rooms (including one devoted to mouse pathology), tissue processing facilities, histology laboratory with four full-time technicians (one devoted to the Core) and an automated Dako immunostainer. In Situ Molecular Pathology Core Lab with a Ventana staining system and NUANCE PLUS InForm computer-based analyses of co-expression of molecular targets, microscopy facilities including a 10-headed microscope with video output for conferences, laser capture microdissection facility and staff, tissue microarray laboratory, mouse imaging facilities including high resolution ultrasound, digital gross and microscopic photography, histomorphometry equipment including a fluorescent microscope and Image Pro Plus analysis software, and mouse surgery facilities. A SharePoint website will facilitate data transfer and sharing between the cores and project staff. These faculty and services will provide a unique resource to the research projects and significantly enhance the quality and validity of the research data.

项目摘要（请参阅说明）：比较病理核心A将为三个科学项目提供集中的病理支持，并代表了小鼠病理学核心的发展，从初始赠款周期中。比较病理学核心的主要目标是在乳腺和乳腺癌病理学方面提供最新的小鼠和人类病理学专业知识。此外，小鼠病理学中的DVM，博士后DOC将执行小鼠死灵（尸检），组织的切割和加工以及在教师病理学家的监督下对组织病理学和特殊污渍的评估。这将确保研究项目的卓越服务和连续性。核心将有6名人员包括三个病理学家（一名医学和两名兽医），医学肿瘤学家（乳腺癌专家），病理学研究员和兼职组织学技术人员。比较病理核心对于确保将小鼠遗传模型转换为人类乳腺癌的特定目的和实验的整合至关重要。这三位病理学家和肿瘤科医生在合作项目上共同努力方面有着悠久的记录，因此在为核心A提供支持方面，也有很好的支持。此外，这三位病理学家和肿瘤学家都与DRS合作。 Ostrowski和Leone在对乳腺癌微环境及其与乳腺癌进展的关系高的手稿上共享作者身份（1，2）。设施将包括四个尸检室（包括一间专门用于小鼠病理学的室），组织加工设施，具有四名全日制技术人员（一名专用于核心）的组织学实验室和一个自动化的Dako免疫染色器。带有Ventana染色系统和细微差别的原位分子病理学核心实验室以及基于计算机的分子靶标共表达的分析，显微镜设施，包括10头显微镜，带有视频输出，激光捕获显微回答设施和工作人员，组织微阵列实验室，鼠标成像设施，包括高分辨率超声，数字总体摄影，数字图和显微照片，包括组织形态计算设备，包括一种荧光显微镜和图像Pro加分析软件以及小鼠手术设施。 SharePoint网站将促进核心和项目人员之间的数据传输和共享。这些教师和服务将为研究项目提供独特的资源，并显着提高研究数据的质量和有效性。