Department of Pathology LSR2 Cytometer

病理科LSR2细胞仪

基本信息

批准号：
6877558
负责人：
FREDERIC Ira PREFFER
金额：
$ 38.9万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-01-01 至 2005-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6877558
关键词：
biomedical equipment purchase cell biology cell differentiation cell sorting flow cytometry

项目摘要

DESCRIPTION (provided by applicant): Funds are requested to purchase a Special Multi-Laser LSR-2 flow cytometer [Becton-Dickinson] to be placed in the PI's new Flow Cytometry Core in the Center for Regenerative Medicine and Technology [CRMT] opening in 2005. The CMRT is one part of an entirely new 150 million-dollar research building presently under construction in downtown Boston, part of Massachusetts General Hospital [MGH]. Dr. Preffer's Core Cytometry Laboratory will be the only one in the entire new research building or the adjoining MGH. If funded, the requested instrument will serve as the analytic centerpiece to an integrated 5-laser FACSDiVa cell sorting system. The Special Multi-Laser LSR-2 is a six laser instrument and the PI is unaware of any commercially available flow cytometer in the greater metropolitan Boston area similar to it. The PI has recently demonstrated ten-color immunofluorescence capacity and feels the requested instrument will further the aims of the named co-investigators in this application. Due to vast improvements and technological advances in cytometry in recent years, the PI's existing LSR-1 is technologically incapable of the level of performance inherent in the requested instrument. The Department of Pathology Flow Cytometry Core laboratory has served the MGH research community since 1978, and is the only one easily available to all investigators at MGH. For this application, the requested instrument will serve a multidisciplinary group of 18 PIs representing 24 NIH funded grants. The laboratory has a highly skilled operator and PI with a combined 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration.

描述（由申请人提供）：要求资金购买特殊的多激光LSR-2流式细胞仪[Becton-Dickinson]，将放置在2005年再生医学和技术中心的PI新的流式细胞仪核心中，该核心于2005年开放。CMRT是全新的15000万个群落的一部分。 [MGH]。 Preffer博士的核心细胞仪实验室将是整个新研究大楼或毗邻MGH的唯一一个。如果资助，请求的仪器将作为集成的5射线FACSDIVA细胞分选系统的分析核心。特殊的多激光LSR-2是一种六激光仪器，PI不知道与它类似的大都市波士顿地区的任何市售流式细胞仪。 PI最近证明了十色免疫荧光能力，并认为所请求的仪器将进一步进一步在本应用程序中指定的共同投资者的目标。近年来，由于细胞仪的巨大改进和技术进步，PI现有的LSR-1在技术上无需该请求仪器固有的性能水平。自1978年以来，病理学流式细胞仪核心实验室一直为MGH研究社区服务，并且是MGH所有研究人员唯一可用的人。对于此申请，所请求的工具将为代表24个NIH资助的赠款的18个PI的多学科小组提供服务。该实验室具有高技能的操作员和PI，具有40年的流式细胞仪经验。在必要时，实验室已经以用户费用，赠款和部门支持的结合来管理和维持自己。该实验室拥有病理部和MGH行政部的财政支持。