Department of Pathology LSR2 Cytometer

病理科LSR2细胞仪

基本信息

批准号：
6877558
负责人：
FREDERIC Ira PREFFER
金额：
$ 38.9万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-01-01 至 2005-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6877558
关键词：
biomedical equipment purchase cell biology cell differentiation cell sorting flow cytometry

项目摘要

DESCRIPTION (provided by applicant): Funds are requested to purchase a Special Multi-Laser LSR-2 flow cytometer [Becton-Dickinson] to be placed in the PI's new Flow Cytometry Core in the Center for Regenerative Medicine and Technology [CRMT] opening in 2005. The CMRT is one part of an entirely new 150 million-dollar research building presently under construction in downtown Boston, part of Massachusetts General Hospital [MGH]. Dr. Preffer's Core Cytometry Laboratory will be the only one in the entire new research building or the adjoining MGH. If funded, the requested instrument will serve as the analytic centerpiece to an integrated 5-laser FACSDiVa cell sorting system. The Special Multi-Laser LSR-2 is a six laser instrument and the PI is unaware of any commercially available flow cytometer in the greater metropolitan Boston area similar to it. The PI has recently demonstrated ten-color immunofluorescence capacity and feels the requested instrument will further the aims of the named co-investigators in this application. Due to vast improvements and technological advances in cytometry in recent years, the PI's existing LSR-1 is technologically incapable of the level of performance inherent in the requested instrument. The Department of Pathology Flow Cytometry Core laboratory has served the MGH research community since 1978, and is the only one easily available to all investigators at MGH. For this application, the requested instrument will serve a multidisciplinary group of 18 PIs representing 24 NIH funded grants. The laboratory has a highly skilled operator and PI with a combined 40 years of well documented experience in flow cytometry. The laboratory has administered and sustained itself on a combination of user fees, grants and departmental backing when necessary. The laboratory has the documented financial support of both the Department of Pathology and MGH administration.

描述（由申请人提供）：需要资金购买一台特殊的多激光 LSR-2 流式细胞仪 [Becton-Dickinson]，并将其放置在再生医学和技术中心 [CRMT] 的 PI 新流式细胞术核心中。 2005 年。CMRT 是目前正在波士顿市中心建设的耗资 1.5 亿美元的全新研究大楼的一部分，该大楼是马萨诸塞州总医院 [MGH] 的一部分。 Preffer 博士的核心细胞计数实验室将是整个新研究大楼或毗邻的 MGH 中唯一的实验室。如果获得资助，所需的仪器将作为集成 5 激光 FACSDiVa 细胞分选系统的分析核心。特殊多激光 LSR-2 是一种六激光仪器，PI 不知道波士顿大都市区有任何与其类似的市售流式细胞仪。 PI 最近展示了十色免疫荧光能力，并认为所需的仪器将进一步实现该应用中指定联合研究人员的目标。由于近年来细胞计数技术的巨大改进和技术进步，PI 现有的 LSR-1 在技术上无法达到所需仪器固有的性能水平。病理学系流式细胞术核心实验室自 1978 年以来一直为 MGH 研究界提供服务，并且是 MGH 唯一一个可供所有研究人员轻松使用的实验室。对于本申请，所请求的工具将服务于由 18 名 PI 组成的多学科小组，代表 24 项 NIH 资助的赠款。该实验室拥有技术精湛的操作员和 PI，他们在流式细胞术方面拥有 40 年的丰富经验。该实验室通过使用费、赠款和必要时部门支持的结合来管理和维持自身。该实验室得到了病理科和麻省总医院管理部门的有据可查的财政支持。