ImageStream X Analyzer 2011

ImageStream X 分析仪 2011

• 批准号: 8247324
• 负责人: FREDERIC Ira PREFFER
• 金额: $ 59.5万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247324
• 关键词: Area; Autoimmune Process; Biological Assay; Boston; Budgets; Cells; Cellular biology; Communicable Diseases; Confocal Microscopy; Data; Dyes; Flow Cytometry; Fluorescence Microscopy; Funding; General Hospitals; Geographic Locations; Grant; Healthcare; Healthcare Systems; Hospitals; Image; Imagery; Immunofluorescence Immunologic; Immunology; Immunophenotyping; Investigation; Laboratories; Lasers; Location; Massachusetts; Microscopy; National Center for Research Resources; Pathology; Population; Principal Investigator; Research; Research Personnel; Sampling; Speed; Stem Cell Research; Technology; United States National Institutes of Health; Universities; Update; Visual; Work; base; cellular imaging; improved; insight; instrument; instrumentation; lens; oncology; operation

DESCRIPTION (provided by applicant): NIH NCRR funds are requested in this resubmission to purchase a 5-laser 2-camera Amnis ImageStreamX with three (20x, 40x, 60x) objective lenses. This new platform is a vastly improved, refined and far more powerful version of the original instrument Amnis introduced approximately eleven years ago. This institutionally supported and expertly supervised instrument will be placed in the principal investigator's existing Department of Pathology Flow Cytometry Core which has been in successful operation for almost 30 years. The requested instrument will serve to fill the gap between the highly detailed (but far more slowly acquired) cellular images obtained by confocal microscopy and the imageless but rapidly acquired data obtained by immunofluorescence-based multiparameter flow cytometry. The ImageStreamX combines these two later technologies, providing a synergistic advantage over each used in tandem. The requested instrument quantifies both the intensity and intracellular location of fluorescent cellular probes and can image at high rates of speed (up to ~2000 cells/sec), allowing our consortium of Massachusetts General Hospital (MGH) investigators to analyze rare sub- populations and highly heterogeneous samples with statistically robust and objective results. This technology will permit some investigations that would otherwise be impossible to perform, to actually be implemented. By combining the speed, sensitivity, and immunophenotyping abilities and capacities of flow cytometry with the detailed imagery and functional insights of microscopy, the requested instrument will be an invaluable addition to represented researchers at both downtown Boston and Charlestown research campuses of MGH, and throughout the greater Partners Health Care System. The instrument utilizes the same dyes and markers employed in fluorescence microscopy and flow cytometry and can perform virtually any standard flow cytometry assay with the added value of quantitative visual inspection and resulting confirmation. The requested instrument will enable 30 investigators representing 77 NIH funded grants to enter into entirely new and useful areas of investigation. There is currently no similar instrumentation at MGH, anywhere in Partners Health Care/Harvard University, or the surrounding geographic region. A wide variety of studies related to cell biology, immunology, autoimmune, infectious disease, oncology and stem cell research will benefit from the placement of this instrumentation in the principal investigator's laboratory. An ImageStreamX demo-unit was placed in the principal investigator's laboratory in February 2010 for one week; pilot data produced from those 5-days is presented in this submission as well as updated data for this 2011 re-submission. The co-investigators in this application represent over 27 million dollars of NIH funded work based at MGH; the entire hospital of approximately 1000 principal investigator's has a NIH budget of approximately $325 million dollars.

描述（由申请人提供）：在此重新提交中请求 NIH NCRR 资金购买具有三个（20x、40x、60x）物镜的 5 激光 2 摄像头 Amnis ImageStreamX。这个新平台是大约 11 年前推出的原始仪器 Amnis 的大幅改进、精致且功能更强大的版本。这种得到机构支持和专家监督的仪器将放置在主要研究者现有的病理学流式细胞术核心中，该核心已成功运行近 30 年。所需的仪器将填补通过共聚焦显微镜获得的高度详细（但获取速度慢得多）的细胞图像与通过基于免疫荧光的多参数流式细胞术获得的无图像但快速获取的数据之间的差距。 ImageStreamX 结合了这两种后来的技术，与同时使用的每种技术相比，具有协同优势。所需的仪器可量化荧光细胞探针的强度和细胞内位置，并且可以高速成像（高达约 2000 个细胞/秒），使我们的马萨诸塞州总医院 (MGH) 研究人员联盟能够分析罕见的亚群和高度异质的样本，具有统计稳健且客观的结果。这项技术将使一些本来无法进行的调查得以实际实施。通过将流式细胞术的速度、灵敏度和免疫表型分析能力与显微镜的详细图像和功能见解相结合，所需的仪器将为麻省总医院波士顿市中心和查尔斯顿研究园区的代表研究人员以及整个医学中心的代表研究人员提供宝贵的补充。更大的合作伙伴医疗保健系统。该仪器采用与荧光显微镜和流式细胞术相同的染料和标记物，几乎可以执行任何标准流式细胞术测定，并具有定量目视检查和结果确认的附加值。所请求的工具将使代表 77 个 NIH 资助项目的 30 名研究人员能够进入全新且有用的研究领域。目前，麻省总医院、Partners Health Care/哈佛大学或周边地理区域都没有类似的仪器。与细胞生物学、免疫学、自身免疫、传染病、肿瘤学和干细胞研究相关的各种研究将受益于在首席研究员实验室中放置该仪器。 2010 年 2 月，一台 ImageStreamX 演示装置被放置在首席研究员的实验室中一周；本次提交的文件中提供了这 5 天产生的试点数据以及 2011 年重新提交的更新数据。本申请中的联合研究人员代表了 NIH 资助的 MGH 工作，金额超过 2700 万美元；整个医院约有 1000 名主要研究者，NIH 的预算约为 3.25 亿美元。