DEPARTMENT OF PATHOLOGY - HIGH SPEED CELL SORTER

病理科 - 高速细胞分选机

基本信息

批准号：
6054241
负责人：
FREDERIC Ira PREFFER
金额：
$ 47.28万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-04-01 至 2001-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6054241
关键词：
biomedical equipment purchase flow cytometry

项目摘要

Funding is requested to purchase a new Cytomation MLS high speed cell softer to replace a 15-year-old EPICS 753 cell sorter. The Flow Cytometry Core in the Department Of Pathology at Massachusetts General Hospital (MGH) has been a continuous resource open to all basic and clinical research scientists requiring flow cytometry since 1978. The laboratory has administered and sustained itself on a combination of user fees and research grants, with departmental backing when necessary. The laboratory has a highly skilled operator and PI with documented expertise in flow cytometry, resulting in a combined 30 years of experience in flow cytometry. The need for a contemporary cell sorter is required to sustain and improve 27 ongoing NIH funded projects from combination of 10 Major and 6 Minor Principal Investigators, described in this application. The requested instrument has specifications that conservatively project an overall tenfold improvement in sorting speed and greatly improved multicolor analytic capacity, compared to the EPICS 753. The purpose of this proposal is to illustrate how the EPICS 753, which was engineered in the late 1970's, has been thoroughly utilized to its utmost capacity but is unable to attain the performance level of the current state-of- the-art instrumentation. Furthermore, Coulter-Beckman no longer services the EPICS 753. It is felt that the options requested for the new instrument are reasonable and warranted by the project summaries submitted by the contributing user groups

要求资金购买新的细胞化MLS高速细胞较软，以取代15岁的Epics 753细胞分隔器。马萨诸塞州综合医院（MGH）病理学系的流式细胞仪核心一直是对自1978年以来需要流式细胞仪的所有基础和临床研究科学家开放的连续资源。实验室已经以用户费用和研究的结合进行了管理和维持赠款，必要时有部门的支持。该实验室具有高技能的操作员和PI，具有流式细胞仪的专业知识，从而获得了30年的流式细胞仪经验。需要对当代细胞分隔器进行维持和改善27个正在进行的NIH资助的项目，从本申请中描述的10名主要和6个次要的主要研究人员组合。所请求的仪器的规格保守地预测了排序速度的总体提高十倍，并且与Epics 753相比，多色分析能力大大提高。该提案的目的是说明在1970年代后期设计的史诗753是如何进行的。被彻底利用达到其最大能力，但无法达到当前最新工具的性能水平。此外，库尔特·贝克曼（Coulter-Beckman）不再为史诗753提供服务。人们认为，新工具要求的选项是合理的，并由贡献用户组提交的项目摘要保证