A LEICA TCS SP CONFOCAL MICROSCOPE FOR WHITNEY LAB

适用于 WHITNEY 实验室的 LEICA TCS SP 共焦显微镜

• 批准号: 6051630
• 负责人: PAUL J LINSER
• 金额: $ 36.06万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051630
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

Technological advances of the last decade have made possible integrated analyses of biological systems never before possible. One of the more striking advances has been the development of microscopic imaging techniques that allow the very high resolution of temporal and spatial relationships of structures and molecules within living and fixed cells and tissues. It is now incumbent on the investigator to describe cell biological processes, not just in reference to what molecules and gene products are involved, but also in terms of how it is that those molecules and gene products relate to one another during cell formation. Recent developments in laser scanning confocal microscopy make such analyses not only possible but required for any state of the art inquisition of cell biological processes. We request funds to purchase a three-laser confocal microscope (Leica TCS SP). The microscope will be shared by the faculty of the Whitney Laboratory, a remote institute of the University of Florida. Six co-PIs representing 7 funded NIH research grants will be the major user group with additional faculty of the Whitney Lab using the faculty when available. We have a common requirement for the high resolution spatial and temporal analyses of a diversity of fundamental cell biological investigations. The research group lead by Barry W. Ache will be investigation ionic events and structural details associated with olfactory processing. William Harvey, Paul Linser and Leonid Moroz together are investigating the molecular physiology of larval mosquitoes, the top- ranked threat to human health worldwide. Robert Greenberg and his associates are investigating the ion channels and physiology of another health threat, schistosomes, the cause of the second most prevalent tropical disease. Roney Laine's research group focuses on investigation of the supramolecular distribution and redistribution of specific mRNA molecules associated with starvation. Many of the proposed experiments require specific optical configurations, time constraints and accessory equipment that are nearly impossible to achieve on a more broadly used facility. Indeed, the only laser scanning confocal microscope available to faculty of the Whitney Lab is a component of the University of Florida's Center for Structural Biology. This facility is the only confocal microscope available to the biological scientists of the entire University of Florida. The extreme limitations that are placed on this widely used microscope by its very broad user requirements are compounded dramatically by the fact that it is 90 miles away from the Whitney Lab. Since the living preparations that are being investigated at the Whitney Lab are main aquatic and/or marine, many of our investigations simply cannot be performed at the University of Florida's Center for Structural Biology, 2 hours away by car.

过去十年的技术进步使对生物系统的综合分析成为可能。最引人注目的进步之一是开发了微观成像技术，这些技术允许在生物和固定细胞和组织中非常高的结构和分子的时间和空间关系分辨率。现在，研究人员有责任描述细胞生物学过程，而不仅仅是指涉及哪些分子和基因产物，而且还指在细胞形成期间这些分子和基因产物之间彼此相关的是如何相关的。激光扫描共聚焦显微镜的最新发展使得这种分析不仅可能发生，而且对细胞生物学过程的任何最先进的宗教裁判所必需。 我们要求资金购买三激光共聚焦显微镜（Leica TCS SP）。显微镜将由佛罗里达大学偏远研究所惠特尼实验室学院共享。六个代表7项资助的NIH研究补助金的共同案例将成为主要用户组，并在可用的情况下使用该教师，并拥有惠特尼实验室的其他教职员工。我们对基本细胞生物学研究多样性的高分辨率空间和时间分析有共同的要求。 Barry W. Ache领导的研究小组将研究与嗅觉处理相关的离子事件和结构细节。威廉·哈维（William Harvey），保罗·林瑟（Paul Linser）和莱昂尼德·莫罗兹（Leonid Moroz）一起研究了幼虫蚊子的分子生理学，这是全世界对人类健康的最高威胁。罗伯特·格林伯格（Robert Greenberg）和他的同事正在调查另一种健康威胁的离子渠道和生理学，即血吸虫，这是第二个最普遍的热带疾病的原因。罗尼·莱恩（Roney Laine）的研究小组着重于研究与饥饿相关的特定mRNA分子的超分子分布和重新分布。许多提出的实验需要特定的光学配置，时间限制和附件设备，这些设备几乎无法在更广泛的设施上实现。确实，惠特尼实验室学院可用的唯一激光扫描共聚焦显微镜是佛罗里达大学结构生物学中心的组成部分。该设施是整个佛罗里达大学的生物科学家可用的唯一共共聚焦显微镜。由于它距离惠特尼实验室（Whitney Lab）90英里，因此，由于其非常广泛的用户需求，其非常广泛的用户需求所面临的极端局限性急剧更加复杂。由于在惠特尼实验室正在调查的生活准备工作是主要的水生和/或海洋，因此我们的许多调查根本无法在佛罗里达大学的结构生物学中心进行，乘汽车2小时。