ANION REGULATION IN LARVAE OF DISEASE VECTOR MOSQUITOES

病媒蚊幼虫中的阴离子调节

• 批准号: 6349905
• 负责人: PAUL J LINSER
• 金额: $ 20.35万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349905
• 关键词: Aedes; Manduca; Xenopus oocyte; bicarbonates; carbonate dehydratase; complementary DNA; enzyme activity; gastrointestinal absorption /transport; gastrointestinal epithelium; hydrogen potassium exchanging ATPase; larva; molecular cloning; nucleic acid sequence

DECSRIPTION (Adapted from the Applicant's Abstract): The long term objective of this project is to isolate, characterize and clone carbonic anhydrase and chloride/bicarbonate anion exchanger from the disease vector mosquito, Aedes aegypti, and the transport model caterpillar, Manduca sexta. The role of theses two enzymes in midgut alkalinzation and ionic homeostasis will be determined and models of anionic buffer regulation will be formulated. The isolated proteinscDNAs will be used to prepare immunological and molecular probes for cellular localization analyses. The hupothesies is that the uniquely high alkalinity of the midgut contents is achieved across an H+ V-ATPase-hyperpolarization membrane by electrophoretic exchange of luminal H+ for cell K+, substrate-driven exchange of luminal Cl- for HCO3-, and voltagew driven removal of H+ to yield CO3--. Since, carbonic anhydrase and anion exchanger inhibition maqy be attractive candidates for novel, environmentally safe, larvacides.

DECSRIPTINT（根据申请人的摘要进行了改编）：长期 该项目的目的是隔离，特征和克隆碳 疾病的赤铁酶和氯化物/碳酸氢盐阴离子交换剂 Vector Mosquito，Aedes Aegypti和Transport Model Caterpillar， Manduca Sexta。这些酶在中肠碱化中的作用 将确定离子稳态和阴离子缓冲液模型 法规将被制定。将使用孤立的蛋白质cDNA 为细胞定位准备免疫学和分子探针 分析。 Hupothesies是，独特的高碱度 Midgut含量是在H+ V-ATPase-Hyperpolarized上实现的 膜通过电泳交换腔H+用于细胞K+的膜， HCO3-和电压驱动的底物驱动的Luminal Cl-交换 去除H+以产生二氧化碳 - 。从那以后，碳酸酐酶和阴离子 交换器抑制Maqy是小说的有吸引力的候选人， 环境安全，幼虫。