Anion Regulation in Larvae of Disease Vector Mosquitoes

病媒蚊幼虫中的阴离子调节

• 批准号: 7384466
• 负责人: PAUL J LINSER
• 金额: $ 30.45万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7384466
• 关键词: Aedes; Alkalinization; Anions; Anopheles gambiae; Biological; Biology; Bioterrorism; Cell physiology; Cells; Culicidae; Depth; Development; Disease; Disease Vectors; Engineering; Epithelial Cells; Food; Gene Expression; Gene Family; Genes; Homeostasis; Human; Individual; Larva; Localized; Maps; Measurement; Midgut; Modeling; Molecular; Numbers; Organism; Pattern; Phenotype; Physiological; Process; Production; Proteins; Regulation; Role; Stomach; Tissues; United States; Viral; West Nile virus; World Health; base; carbonate dehydratase; gastrointestinal epithelium; member; microbial; novel; pathogen; vector; vector mosquito

DESCRIPTION (provided by applicant): The long term objective is to characterize key functionalities of the epithelial cells of the larval mosquito gut as these cellular functions influence and regulate the anionic basis of alkalinization of the gut lumen. A detailed understanding of how gut epithelial cells produce the remarkable and biologically unique pH extremes (i.e. > 10.5) that drive the digestive process will provide new avenues for the development of environmentally safe and specific larvacides. Two specific gene families have been targeted as they have central roles in anion production and transport in the gut alkalinization process: carbonic anhydrases and transmembrane anion transporters. This project will produce molecular and physiological characterizations of members of these two gene families. Their distributions in the tissue and specific roles in larval mosquito gut alkalinization will be defined. Specific cellular phenotypes throughout the gut will be defined and the role of each in the alkalinization process assessed. AIM 1 will examine the expression of multiple carbonic anhydrases. AIM 2 will define and characterize members of the anion transporter gene family. AIM 3 will define the cellular distributions of carbonic anhydrases and anion transporters in the gut and as functions of larval development. AIM 4 will produce a global analysis of gene expression in the specific functional domains of the larval mosquito gut identifying key functionalities which define the gut domains. AIM 5 will bring the localization of specific gene products together with physiological measurements of the activity of individual cells to produce a cell-specific and spatial analysis of anion dynamics in the gut epithelium. As mosquitoes are the number one threat to human health world wide and recognized as potential agents for bioterrorism, the development of new strategies for control based on unique aspects of their biology (i.e. gut alkalinization) has important potential.

描述（由申请人提供）：长期目标是表征幼虫蚊子肠道上皮细胞的关键功能，因为这些细胞功能会影响和调节肠道腔碱化的阴离子基础。对肠道上皮细胞如何产生驱动消化过程的显着和生物学独特的极端pH极端（即> 10.5）的详细理解将为开发环境安全和特定的幼虫的发展提供新的途径。两个特定的基因家族在肠道碱化过程中具有中心作用，因为它们在阴离子的产生和运输中具有中心作用：碳赤霉酶和跨膜阴离子转运蛋白。该项目将产生这两个基因家族成员的分子和生理特征。它们在组织中的分布以及在幼虫蚊子肠林化中的特定作用将定义。将定义整个肠道中的特定细胞表型，并评估每个肠道的作用在碱化过程中的作用。 AIM 1将检查多种碳酸酐酶的表达。 AIM 2将定义和表征阴离子转运蛋白基因家族的成员。 AIM 3将定义肠道和阴离子转运蛋白的细胞分布，并作为幼虫发育的功能。 AIM 4将在幼虫蚊子肠道的特定功能结构域中产生全局分析，从而识别定义肠道结构域的关键功能。 AIM 5将将特定基因产物的定位以及对单个细胞的活性进行生理测量，以产生肠道上皮中阴离子动力学的细胞特异性和空间分析。由于蚊子是全世界人类健康的第一威胁，并被认为是生物恐怖主义的潜在推动者，因此基于其生物学独特方面（即肠道碱化）的新策略的发展具有重要的潜力。