MASS SPECTROMETER

质谱仪

基本信息

批准号：
6291665
负责人：
WOLFGANG H FISCHER
金额：
$ 26万
依托单位：
SALK INSTITUTE FOR BIOLOGICAL STUDIES
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-04-15 至 2002-04-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6291665
关键词：
biomedical equipment purchase mass spectrometry

项目摘要

This is a request for a time-of-flight (TOF) mass spectrometer. The instrument will play a pivotal role in the research projects of many investigators at the Salk Institute and researchers at other academic institutions (see letters of support). We will obtain accurate mass and structural information on novel hormones, toxins, and growth factors and characterize the sites and nature of post-translational modifications in molecules of known primary structure. A group of 10 investigators has been identified whose research is dependent on analyses only possible with the requested instrument. The sensitivity of the instrument is essential to our current attempts to characterize novel peptides and proteins. Results obtained on a variety of unstable peptides illustrate the increased sensitivity available with pulsed ion extraction and the laser optics on the requested instrument compared with continuous extraction and the more rudimentary analysis currently available. The instrument will also be vital for identifying the site of phosphorylation in phosphoproteins where either the low level of phosphorylation or the deleterious effects of introducing a radioactive tracer prohibit conventional electrophoresis and radioactive chemical sequencing protocols. By analyzing the hydrolyzed phosphoprotein, we will increase the sensitivity of intact mass analysis while the use of fully automated precursor selection and PSD will allow rapid MS2 analysis of the phosphopeptide to help identify the site of phosphorylation when more than one potential site of phosphorylation exists in the peptide. The mass accuracy and resolution available with the upgraded instrument will be extremely valuable for the identification of hydrolysis fragments generated from 'unknown' proteins. The instrument will be amenable to a proposed approach of measuring a peptide both prior to and after carrying out reduction and thereby enabling PSD analysis of disulfide bridge-containing peptides. Other instruments already available at the Salk Institute include a double focusing magnetic sector mass spectrometer with both liquid secondary ionization and laser desorption ionization sources, an electrospray ion trap mass spectrometer, and gas phase sequencers with PTH analyzers. The requested upgraded TOF mass spectrometer is needed to complement the strong classical Edman sequencing approach at the Salk Institute. The instrument will be located in the MS Facility. A significant institutional commitment will ensure that this instrument is operated and maintained by trained personnel. All measurements will be made under the supervision of A. Craig.

这是飞行时间（TOF）质谱仪的要求。该工具将在Salk Institute的许多研究人员和其他学术机构的研究人员的研究项目中发挥关键作用（请参阅支持信）。我们将获得有关新型激素，毒素和生长因子的准确质量和结构信息，并表征已知主要结构分子的翻译后修饰的位置和性质。已经确定了一组10名调查人员的研究，其研究仅取决于所请求的工具的分析。该仪器的敏感性对于我们目前的表征新型肽和蛋白质的尝试至关重要。与连续提取相比，在多种不稳定肽上获得的结果说明了脉冲离子提取和激光光学元件的灵敏度的提高，并且目前可用的更基本的分析。该仪器对于鉴定磷酸蛋白中的磷酸化位点至关重要，在磷酸蛋白中，低水平的磷酸化或引入放射性示踪剂禁止传统电泳和放射性化学测序方案的有害作用。通过分析水解的磷酸蛋白，我们将提高完整质量分析的敏感性，而使用全自动的前体选择和PSD将允许对磷酸肽的快速分析，当在肽中存在磷酸化的一个潜在的位点时，可以帮助识别磷酸化的位点。升级仪器可用的质量精度和分辨率对于鉴定从“未知”蛋白产生的水解片段非常有价值。该仪器将适合于在还原之前和之后测量肽的建议方法，从而实现PSD分析含二硫桥梁的肽。 Salk Institute已经可用的其他仪器包括具有液体次级电离和激光解吸电离源的双聚焦磁性扇形质谱仪，电喷雾离子TRAP质谱仪和带PTH分析仪的气相测序仪。需要升级的TOF质谱仪升级，以补充Salk Institute的强大古典Edman测序方法。该仪器将位于MS设施中。一项重大的机构承诺将确保该工具由训练有素的人员运作和维护。所有测量将在A. Craig的监督下进行。