CORE--CHARACTERIZATION OF PROTEINS/RECOMBINANT PROTEINS

核心——蛋白质/重组蛋白质的表征

• 批准号: 6439480
• 负责人: WOLFGANG H FISCHER
• 金额: $ 19.74万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6439480
• 关键词: biomedical facility; follistatin; inhibin; mass spectrometry; protein purification; protein sequence; recombinant proteins

Two essential components of the Projects of this Program will be supported by this Core. The first is the characterization of novel or modified peptides and proteins by Edman based chemical sequencing and mass spectrometric techniques. The second is the expression and purification of proteins that are required by the Projects for biological and biochemical investigations. Protein characterization is an essential part of this Program. The Core provides both state-of-the-art instrumentation and highly trained investigators and support personnel to carry out these characterizations. The Core will characterize novel peptides, proteins or post-translational modifications of known proteins being studied by Program investigators and in particular, mediators involved in actin and inhibin signaling. In addition, we will confirm the sequence and post-translational modifications of recombinant proteins that the Projects generate for use in biological and crystallization studies. Several projects require large quantities of proteins such as activin, activin mutants, inhibin, and soluble fragments of receptors. The Core will generate these proteins by expressing the genes in a suitable system (e.g. baculovirus/insect, mammalian, or yeast cells). The proteins will be purified and characterized using appropriate discriminatory analytical techniques.

该计划的项目的两个基本组成部分将得到该核心的支持。首先是通过基于Edman的化学测序和质谱技术对新型或改良的肽和蛋白质的表征。第二个是生物学和生化研究所需的蛋白质的表达和纯化。蛋白质表征是该程序的重​​要组成部分。核心既提供了最先进的仪器，也提供了训练有素的调查人员，也提供了支持人员来执行这些特征。该核心将表征程序研究者，尤其是参与肌动蛋白和抑制蛋白信号传导的介质研究的已知蛋白质的新型肽，蛋白质或翻译后修饰。此外，我们将确认项目用于生物学和结晶研究的重组蛋白的序列和翻译后修饰。几个项目需要大量蛋白质，例如激活素，激活素突变体，抑制素和可溶性受体片段。核心将通过在合适的系统（例如杆状病毒/昆虫，哺乳动物或酵母细胞）中表达基因来产生这些蛋白质。蛋白质将使用适当的歧视性分析技术进行纯化和表征。