AB-4000 QTRAP MASS SPECTROMETER

AB-4000 QTRAP 质谱仪

• 批准号: 6877428
• 负责人: NATALIE G. AHN
• 金额: $ 49.66万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-15 至 2006-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877428
• 关键词: biomedical equipment purchase; data collection; liquid chromatography mass spectrometry; posttranslational modifications; protein sequence; proteomics

DESCRIPTION (provided by applicant): The goal of this proposal is to support innovative research in functional proteomics for investigators at the University of Colorado, Boulder (CU-Boulder). Funds are requested to purchase an integrated system for shotgun proteomic profiling by multidimensional LC/MS/MS and profiling protein post-translational modifications (PTMs), building on a novel approach to more sensitive and accurate proteomic profiling, as well as new computational tools for data reduction. The system includes a ABI-4000 QTrap mass spectrometer capable of rapid scanning and high mass accuracy for efficient data collection and two HPLCs. Advantages of the requested instrument are: 1) higher quality MS/MS spectra and higher percentage of usable data generated in each file, 2) superior performance with phosphopeptide sequencing, and 3) no requirement for a large computer cluster to support data collection. The user group includes protein chemists, geneticists, cell biologists, computational scientists, and clinicians, with expertise in mass spectrometry, signal transduction, cancer biology, mammalian and microbial cell regulation, genomics, evolutionary diversity metabolic pathways, computational modeling, and databasing. The projects are highly complementary, and represent research along major themes of (1) eukaryotic signal transduction, cellular regulation, and disease progression, and (2) microbial diversity and mechanisms of host-pathogen interactions. The system will be managed by the Central Analytical Facility, which will provide technical, computational, and bioinformatics support needed for users to carry out proteomic profiling experiments. This facility provides investigators throughout the university with open access to departmental and investigator-owned instruments, and has 10 years of experience as a training facility that emphasizes handson student use, in addition to traditional service arrangements. A Facility Manager and MS Technician will be responsible for daily operation and maintenance of the system; both will be supervised by a faculty member serving as Facility Director. The cost of instrument operation, service contracts, and partial support for facility staff will be recovered through user fees. An internal advisory committee consisting of faculty users and non-users will ensure equitable distribution of instrument time and fairness in fee schedules for use of the instrument.

描述（由申请人提供）：该提案的目标是支持科罗拉多大学博尔德分校（CU-Boulder）的研究人员在功能蛋白质组学方面的创新研究。需要资金购买一个通过多维 LC/MS/MS 进行鸟枪法蛋白质组分析和分析蛋白质翻译后修饰 (PTM) 的集成系统，该系统建立在一种更灵敏、更准确的蛋白质组分析新方法以及新的计算工具的基础上数据减少。该系统包括一台 ABI-4000 QTrap 质谱仪，能够快速扫描和高质量准确度，以实现高效的数据收集和两个 HPLC。所需仪器的优点是：1) 更高质量的 MS/MS 谱图和每个文件中生成的可用数据百分比更高，2) 磷酸肽测序的卓越性能，3) 不需要大型计算机集群来支持数据收集。用户组包括蛋白质化学家、遗传学家、细胞生物学家、计算科学家和临床医生，拥有质谱、信号转导、癌症生物学、哺乳动物和微生物细胞调控、基因组学、进化多样性代谢途径、计算建模和数据库方面的专业知识。这些项目具有高度互补性，代表了以下主要主题的研究：(1) 真核信号转导、细胞调节和疾病进展，以及 (2) 微生物多样性和宿主-病原体相互作用机制。 该系统将由中央分析设施管理，该设施将为用户进行蛋白质组学分析实验提供所需的技术、计算和生物信息学支持。该设施为整个大学的研究人员提供了开放使用部门和研究人员拥有的仪器的机会，并且作为培训设施拥有 10 年的经验，除了传统的服务安排外，还强调学生的实际使用。设施经理和MS技术员将负责系统的日常操作和维护；两者都将受到担任设施主任的教员的监督。仪器操作、服务合同和对设施工作人员的部分支持的成本将通过使用费来收回。由教师用户和非用户组成的内部咨询委员会将确保仪器时间的公平分配以及仪器使用费用表的公平性。