LC-MS/MS FOR HIGHLY SENSITIVE BIOMARKER DETECTION

用于高灵敏度生物标志物检测的 LC-MS/MS

• 批准号: 6877300
• 负责人: JAMES A SWENBERG
• 金额: $ 41.84万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2007-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877300
• 关键词: DNA damage; biomarker; biomedical equipment purchase; high performance liquid chromatography; liquid chromatography mass spectrometry; protein quantitation /detection

DESCRIPTION (provided by applicant): The Biomarker Mass Spectrometry Facility supports research on the UNC-Chapel Hill Campus by providing mass spectrometric identification, characterization and quantitation of biomarkers of exposure. The Biomarker Mass Spectrometry Facility seeks funding to acquire an ultra-high performance bench top triple quadrupole LC-MS/MS system. Our need for the requested instrument is two-fold. Currently the instruments operated by the Biomarker MS Facility are operating around the clock (except for instrument down-time and maintenance) and analyses require four or more weeks advance scheduling. The current volume of demand for the Facility's analytical services exceeds our capacity, resulting in delays in scheduling analyses and leaving no leeway for new projects that are being initiated as assays for DNA damage which we have developed are being transferred to other users on campus. Our other pressing need is for higher-sensitivity analysis than can be provided with the current instrumentation. Because we are measuring analytes which are present at low levels in samples such as human tissue which are available in very limited quantity only, we need sensitivity of detection well beyond what can be achieved with the instruments currently operating in the facility. We therefore seek an instrument with LC-MS/MS, high-throughput capability and high sensitivity. We also need this instrument to accept nano and capillary LC input. This instrument will support research into cisplatin DNA adducts, lipid composition, molecular dosimetry of DNA adducts derived from butadiene, ethylene oxide, propylene oxide and isoprene, and studies on choline metabolism, cancer causality and susceptibility, DNA damage and repair, molecular epidemiology, exposure assessment, and other healthrelated topics. The instrument of choice is an Applied Biosystems/MDS Sciex API 4000 interfaces with an LC Packings (Dionex) capillary/nanoflow high pressure liquid chromatography system. This instrument will be installed in an established and productive shared-use facility staffed by experienced personnel, where it will support the research of ten holders of eleven current or pending R01 and U01 NIH grants.

描述（由申请人提供）：生物标志物质谱设施通过提供质谱识别，表征和定量暴露的生物标志物来支持对UNC教堂山校园的研究。生物标志物质谱设施寻求资金来获得超高的性能台上三倍四极杆LC-MS/MS系统。我们对请求的仪器的需求是两个方面。目前，生物标志物MS设施运行的仪器正在全天运行（仪器停机和维护除外），分析需要提前四个或更长时间。该设施分析服务的目前需求量超过了我们的能力，从而导致安排分析的延迟，并且没有为新项目的余地留下余地，这些新项目作为我们开发的DNA损害的测定法已转移给校园的其他用户。我们的另一个紧迫需求是对当前仪器所提供的更高敏感性分析。因为我们正在测量在人体组织等样品中以低水平的水平存在的分析物，这些分析物仅以非常有限的量获得，因此我们需要远远超出该工具中当前在设施中运行的仪器所能实现的敏感性。因此，我们寻求具有LC-MS/MS，高通量能力和高灵敏度的仪器。我们还需要该仪器来接受纳米和毛细管LC输入。该仪器将支持对顺铂DNA加合物的研究，脂质组成，源自丁二烯，氧化乙烷，氧化丙烯和异丙烯的DNA加合物的分子剂量测定，以及胆碱代谢的研究，癌症捕获性和易感性和易感性，DNA损伤和维修，分子epporsigry，以及其他评估，以及其他。选择的仪器是带有LC包装（Dionex）毛细管/纳米流量高压液相色谱系统的Ap​​plied Biosystems/MDS Sciex API 4000接口。该工具将安装在经验丰富的人员配备的既定且富有成效的共享用途设施中，在那里它将支持十个当前或未决R01和U01 NIH赠款的十个持有人的研究。