Agilent 6490 LCMS Triple Quadrupole Mass Spectrometer with 1260 Infinity Chip Cub

配备 1260 Infinity Chip Cub 的 Agilent 6490 LCMS 三重四极杆质谱仪

• 批准号: 8247540
• 负责人: JAMES A SWENBERG
• 金额: $ 39.7万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247540
• 关键词: Biological Markers; Core Facility; Detection; Ecology; Environmental Engineering technology; Equipment; Funding; High Pressure Liquid Chromatography; Marketing; Mass Spectrum Analysis; North Carolina; Research Personnel; Sampling; Series; System; United States National Institutes of Health; Universities; instrument; instrumentation; interest; mass spectrometer

DESCRIPTION (provided by applicant): We are requesting funding to purchase a versatile, high sensitivity high-throughput system consisting of an HPLC-chip interfaced to a triple quadrupole mass spectrometer. This instrument will be located in the Biomarker Mass Spectrometry Facility Core in the Department of Environmental Sciences and Engineering of the University of North Carolina at Chapel Hill (UNC-CH), which is a shared facility that serves researchers from the entire university and beyond. This instrumentation will provide us with at least an order of magnitude higher sensitivity than the existing instrumentation. This will allow detection and quantitation of analytes that are present at levels too low for our current instrumentation, and/or where the total amount of sample available precludes analysis with our current instrumentation. The equipment of choice is an Agilent 1200 series nanoflow HPLC interfaced via a 1260 Infinity Chip Cube with an Agilent 6490 LCMS Triple Quadupole mass spectrometry system. This combination is the most sensitive LC-MS/MS on the market, and will allow quantitation of biomarkers of interest with the smallest mass and volume of sample. The Chip Cube interface is also very well suited for use in a shared instrument facility where trace-level analysis is very easily compromised by cross-contamination. This equipment will support nine NIH- funded researchers. PUBLIC HEALTH RELEVANCE: The Biomarker Mass Spectrometry Facility provides accessible routine service for measuring small molecules and biomarkers to the University of North Carolina at Chapel Hill biomedical research community. The LCMS/MS system requested for the Biomarker Mass Spectrometry Facility will enable the Facility to extend by at least an order of magnitude the sensitivity of assays for small molecules, DNA adducts, DNA-protein crosslinks, and other biomarkers in complex biological matrices. This will enhance studies by nine NIH-funded researchers at the UNC-CH School of Public Health and School of Medicine, including three physicianscientists, who address critical health problems related to cancer, cystic fibrosis and other respiratory disease and drug abuse.

描述（由申请人提供）：我们要求资金购买一种多功能，高灵敏度的高通量系统，该系统由连接到三倍四倍体质谱仪的HPLC-CHIP组成。该乐器将位于北卡罗来纳大学Chapel Hill（UNC-CH）的环境科学与工程系的生物标志物质谱设施核心，该设施是一个共享设施，为整个大学及其他地区的研究人员提供服务。该仪器将为我们提供至少比现有仪器高的数量级。这将允许对我们当前仪器的水平过低的分析物进行检测和定量，以及/或在我们当前的仪器中可用的样本总量排除分析。选择的设备是通过1260 Infinity Chip Cube与Agilent 6490 LCMS Triple三倍四倍体质谱系统连接的Agilent 1200系列Nanoflow HPLC HPLC。这种组合是市场上最敏感的LC-MS/MS，它将允许用最小的质量和样品量定量感兴趣的生物标志物。芯片立方体界面也非常适合在共享仪器设施中使用，在共享仪表设施中，痕量级别的分析很容易被交叉污染所损害。该设备将支持9位NIH资助的研究人员。 公共卫生相关性：生物标志物质谱设施提供了可访问的常规服务，以测量北卡罗来纳大学教堂山生物医学研究社区的小分子和生物标志物。要求使用生物标志物质谱设施的LCMS/MS系统将使该设施至少延伸到一个数量级的顺序，该测定法对小分子，DNA加合物，DNA蛋白质交联，DNA蛋白交联和其他生物标志物的敏感性在复杂的生物学基质中。这将增强UNC-CH公共卫生学院和医学院的9名NIH资助的研究人员的研究，其中包括三位医生科学家，他们解决了与癌症，囊性纤维化以及其他呼吸道疾病和其他药物滥用有关的关键健康问题。