X RAY DIFFRACTION EQUIPMENT FOR MACROMOLECULES

大分子X射线衍射设备

• 批准号: 2040315
• 负责人: WILLIAM L DUAX
• 金额: $ 15.57万
• 依托单位: HAUPTMAN-WOODWARD MEDICAL RESEARCH INST
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2040315
• 关键词: X ray crystallography; biomedical equipment purchase; macromolecule

The goal of this proposal is to provide partial funding for a second X-ray area detector for use in the Molecular Biophysics Dept. of the Hauptman-Woodward Medical Research Institute, and for the refurbishing and modification of the existing Rigaku R-Axis II. This equipment is to be shared by a major user group consisting of six principal investigators who are conducting research funded by the PHS and a number of projects funded by other means. The requested funds will be used to purchase a Rigatu ~UltraX" rotating anode generator, an ADSC four module CCD detector system, focusing mirrors, K goniostat and low temperature device, and a dedicated SGI workstation. The presence of a second detector will permit the modification of the existing R-Axis II, which is equipped with a graphite monochromator, to unable routine target changes. The addition of the monochromator pivot and three-axis readout and the acquisition of a Cr target win allow target changes while minimizing down time. The instrument can then be used for normal data collection (Cu), and to exploit anomalous scattering techniques in direct methods, maximizing the anomalous signal by using the longest wavelength practical (Cr). Additionally, the image plates, laser, bearings, belts and Be window, which are nearing the end of their expected life, will be replaced. An internal advisory committee consisting of the Institute's President, Executive Vice-President-- Research, and the Head of the Molecular Biophysics Dept. will have overall responsibility for the equipment, and the day-to-day management will be provided by the staff scientist who presently supervises the Institute's shared diffraction facilities, assisted by two other members of the department's scientific staff. The projects to be served by this equipment include the study of steroid metabolizing enzymes, steroid transport proteins, ion transport antibiotics, dihydrofolate reductase/inhibitor complexes, transthyretin complexes, estrogen aromatase and its complex with a monoclonal antibody, folylpolyglutamate synthetase, xylan esterases, and bovine duodenase, as well as the research on new methods of phase determination. The total cost of the acquisition of this equipment and modification of existing equipment will be reduced by an amount of $230,000 already obtained from private sources for the purpose of expanding the protein diffraction facilities.

该提案的目标是为第二个项目提供部分资金 X射线面探测器，用于分子生物物理部 豪普特曼-伍德沃德医学研究所，以及 对现有 Rigaku R-Axis II 进行翻新和改造。这 设备将由六人组成的主要用户组共享 正在进行由 PHS 资助的研究的主要研究人员 以及一些通过其他方式资助的项目。要求的资金 将用于购买 Rigatu ~UltraX" 旋转阳极发电机， ADSC 四模块 CCD 探测器系统、聚焦镜、K 测角仪和低温装置，以及专用的 SGI 工作站。第二个探测器的存在将允许 对现有 R 轴 II 进行修改，该 R 轴 II 配备了 石墨单色仪，无法常规改变目标。这 添加单色仪枢轴和三轴读数以及 获得 Cr 目标胜利允许目标变化，同时最小化 停机时间。然后仪器即可用于正常数据采集 （Cu），并利用反常散射技术直接 方法，通过使用最长的来最大化异常信号 实用波长（Cr）。此外，图像板、激光、 轴承、皮带和 Be 窗口，它们的使用寿命已接近尾声 预计寿命，将被更换。内部顾问委员会 成员包括研究所所长、执行副所长—— 研究和分子生物物理学系主任将有 全面负责设备和日常工作 管理工作将由目前在职科学家提供 监督研究所的共享衍射设施，并由 该部门的另外两名科学人员。项目 该设备的服务范围包括类固醇的研究 代谢酶、类固醇转运蛋白、离子转运 抗生素、二氢叶酸还原酶/抑制剂复合物、 转甲状腺素蛋白复合物、雌激素芳香酶及其复合物 单克隆抗体、叶酰聚谷氨酸合成酶、木聚糖酯酶、 和牛十二指肠酶以及相新方法的研究 决心。购置该设备的总成本 现有设备的改造将减少一定数量 已从私人来源获得 230,000 美元，用于 扩大蛋白质衍射设施。