INSTRUMENTATION FOR SHARED X-RAY FACILITY

共享 X 射线设备的仪器

• 批准号: 2040590
• 负责人: EDWARD ARNOLD
• 金额: $ 12.06万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2040590
• 关键词: X ray crystallography; biomedical equipment purchase; computer data analysis; cryoscience; cryostat; intermolecular interaction; macromolecule; structural biology

A group of four co-investigators and their research groups at Rutgers University and the University of Medicine and Dentistry of New Jersey operate a shared X-ray crystallographic facility. Edward Arnold, Helen Berman, Millie Georgiadis , and Ann Stock are studying crystallographic structures of proteins, nucleic acids, and protein-nucleic acid complexes that span a wide range of biomedically important systems. Funds are requested to purchase an image plate X-ray detector system that will complement the currently saturated data collection resources in the shared X-ray facility (consisting of a Xuong-Hamlin multiwire area detector system and a Rigaku R-Axix II image plate detector system). Over 30 scientists from the four major groups regularly use the shared facility and the structural projects involve collaborations with other highly productive biomedical researchers. The additional detector system requested, the Rigaku R-Axis IV image plate detector and scanner, would enhance the capabilities of the shared X-ray laboratory not only by increasing data collection access time for each of the groups, but also by permitting 1)collection of higher resolution data from both strongly and weakly diffracting crystals and 2) more efficient collection of data from crystals with large unit cells. Funds are also requested to purchase a liquid nitrogen crystal cryostat system and a computer workstation to upgrade the Xuong-Hamlin detector system to optimize its utility to the co-investigators. The outstanding support staff of the shared X-ray laboratory will assist in installation of the new instrument, training and maintenance, and continued operation of the facility. The sponsoring institutions (Rutgers University, the University of Medicine and Dentistry of New Jersey, the Center for Advanced Biotechnology and Medicine, and the Waksman Institute) have contributed seed funding ($40,000) for partial support of the capital costs and are committed to continuing to pay the operations and maintenance costs of the shared facility.

罗格斯大学的四位联合研究人员及其研究小组 大学和新医学和牙科大学 泽西岛运营着一个共享的 X 射线晶体学设施。 爱德华 阿诺德、海伦·伯曼、米莉·乔治亚迪斯和安·斯托克 研究蛋白质、核酸等的晶体结构 蛋白质-核酸复合物的范围广泛 生物医学上重要的系统。 需要资金购买 图像板 X 射线探测器系统将补充当前的 共享 X 射线设施中的数据收集资源饱和 （由 Xiong-Hamlin 多线区域探测器系统和 Rigaku R-Axix II 图像板检测器系统）。 超过30位科学家 来自四个主要群体的人定期使用共用设施和 结构项目涉及与其他高生产力的合作 生物医学研究人员。 要求额外的探测器系统， Rigaku R-Axis IV 图像板探测器和扫描仪，将 增强共享X射线实验室的能力不仅通过 增加每个组的数据收集访问时间，而且 通过允许 1）从双方收集更高分辨率的数据 强和弱衍射晶体，2) 效率更高 从具有大晶胞的晶体中收集数据。 资金还有 要求购买液氮晶体低温恒温器系统和 用于升级Xuong-Hamlin探测器系统的计算机工作站 优化其对联合研究者的效用。 杰出的 共享 X 射线实验室的支持人员将协助安装 新仪器的使用、培训和维护，并继续 设施的运营。 赞助机构（罗格斯大学 大学，新泽西医学和牙科大学， 先进生物技术和医学中心以及 Waksman Institute）为以下项目提供了种子资金（40,000 美元）： 部分支持资本成本并致力于继续 支付共享设施的运营和维护费用。