Macromolecular X-ray Structure Facility Instrumentation

高分子X射线结构设备仪器

基本信息

批准号：
6578685
负责人：
ANDREW D MESECAR
金额：
$ 34.64万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-04-01 至 2005-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6578685
关键词：
X ray crystallography biomedical equipment purchase data collection methodology /evaluation macromolecule monitoring device structural biology

项目摘要

DESCRIPTION (provided by applicant): A state-of-the-art imaging plate area detector with variable 20 stage, low-temperature cooling apparatus, and confocal multilayer optics will be purchased to replace older instrumentation. It will be used to collect x-ray diffraction data from single crystals on a rotating-anode x-ray generator equipped with a Cu target. The unit will be used to expand and enhance the capabilities of the saturated multi-user, macromolecular, single crystal, x-ray diffraction facility in the Molecular Biology Research Building (MBRB) at the University of Illinois at Chicago (UIC). This facility, the UIC/RRC (Research Resources Center) Macromolecular Structure Facility (MSF), is located on the west medical campus, and currently supports seven (Gettins, Jeffery, Johnson, Lavie, Matsumura, Mesecar, Volz) major research groups, including two NIH program grants and one NIH center. Approximately 80% of the instrument time will be utilized by NIH-funded researchers, and the remaining time will be available for other researchers on the UIC campus, in the Chicago area, and for a collaborator in North Carolina. The present research facility was established four years ago, and is used to augment courses in Medicinal Chemistry, Pharmacognosy, Microbiology, Biochemistry, and Pharmaceutical Biotechnology. Access to the instrumentation by new users both on and off campus has been governed by an administrative oversight and advisory committee including campus researchers and RRC personnel. The two area detectors and optics now in operation are heavily used, and are somewhat dated and aging, resulting in substantial downtime and increased maintenance. The Siemens multi-wire area detector, that will be replaced, has no low-temperature cooling system, and is therefore unsuitable for cryo-stored or unstable samples. The new detector and state-of-the-art optics system combination will allow for faster and more accurate in-house data collection on both routine and difficult samples including those with long cell axes, small crystal size, or poor diffraction, as well as provide for faster and more thorough screening of crystals in preparation for synchrotron time. The increased use of the UIC/RRC MSF facility reflects the growing importance of structural biology as a major component in research and teaching at the University of Illiniois at Chicago. The growth in structural biology research at UIC is being fostered by the University Administration and the structural biology community, and is reflected in the establishment of the Center for Pharmaceutical Biotechnology, and most recently the establishment of the Center for Structural Biology.

描述（由申请人提供）：将购买具有可变20级，低温冷却设备和共聚焦多层光学元件的最先进的成像板区域检测器，以取代较旧的仪器。它将用于从配备有CU目标的旋转轴X射线发生器上的单晶收集X射线衍射数据。该单元将用于扩展和增强伊利诺伊州芝加哥大学（UIC）的分子生物学研究大楼（MBRB）中饱和的多用户，大分子，单晶，X射线衍射设施的能力。该设施，UIC/RRC（研究资源中心）大分子结构设施（MSF）位于西部医疗校园，目前支持七个（Gettins，Jeffery，Johnson，Lavie，Lavie，Matsumura，Matsumura，Mesecar，Mesecar，Mesecar，Mesecar，volz，volz，volz），包括两个NIH计划Grants Grants Grants和一个NIH HIH中心。 NIH资助的研究人员将使用大约80％的仪器时间，剩余时间将用于UIC校园，芝加哥地区的其他研究人员以及北卡罗来纳州的合作者。本研究设施成立于四年前，用于增加药物化学，药物学，微生物学，生物化学和药物生物技术的课程。校园内外的新用户可以访问该仪器，包括校园研究人员和RRC人员在内的行政监督和咨询委员会的管辖。目前正在运行的两个区域探测器和光学器件已大量使用，并且有些过时和老化，从而导致大量停机时间和增加的维护。西门子的多线区域检测器将被替换，没有低温冷却系统，因此不适合冷冻储存或不稳定的样品。新的探测器和最先进的光学系统组合将允许在常规和困难的样本上收集更快，更准确的内部数据，包括那些具有长轴轴，小晶体尺寸或较差的衍射量的样本，并提供更快，更彻底的晶体筛选，以准备同步时间。 UIC/RRC MSF设施的增加使用反映了结构生物学作为芝加哥伊利诺伊大学研究和教学的主要组成部分的重要性。 UIC的结构生物学研究的增长正在大学管理和结构生物学界促进，并反映在建立药物生物技术中心，以及最近建立结构生物学中心。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Evaluating the 3C-like protease activity of SARS-Coronavirus: recommendations for standardized assays for drug discovery.

DOI：
10.1016/j.virusres.2007.02.015
发表时间：
2008-04
期刊：
VIRUS RESEARCH
影响因子：
5
作者：
Grum-Tokars, Valerie;Ratia, Kiira;Begaye, Adrian;Baker, Susan C.;Mesecar, Andrew D.
通讯作者：
Mesecar, Andrew D.

Structural basis for catalysis of a tetrameric class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis.

DOI：
10.1016/j.jmb.2009.01.003
发表时间：
2009-03-06
期刊：
JOURNAL OF MOLECULAR BIOLOGY
影响因子：
5.6
作者：
Pegan, Scott D.;Rukseree, Kamolchanok;Franzblau, Scott G.;Mesecar, Andrew D.
通讯作者：
Mesecar, Andrew D.

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.