MUCOSAL IMMUNITY & PROTECTION

粘膜免疫

• 批准号: 6219642
• 负责人: MARNIX L BOSCH
• 金额: $ 12.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6219642
• 关键词: AIDS; Mammalia; animal tissue; communicable diseases; immunology; inflammation; lymphatic system; microorganism; vaccines; virus

We have established immunological assays to assess cellular immunity at mucosal surfaces and in the blood. Using these assays we can show that macaques that are infected with SHIV respond to SHIV antigens in vitro by making gamma-interferon. This response is stronger in the gut than in the periphery. We also used these methods to test macaques immunized with autologous dendritic cells that were transfected with and HIV-1 provirus mutated in the integrase gene. These animals showed a response similar to the infected animals, and we determined that, as expected, the responding cells were in fact CD69+ activated T cells. In addition we have shown that these animals have a classic cytotoxic T lymphocyte response to HIV-1 antigens. FUNDING NIH grants RR00166 and AI26503.

我们已经建立了免疫学测定以评估细胞 粘膜表面和血液中的免疫力。 使用这些测定法 可以证明感染了Shiv的猕猴对Shiv做出了反应 通过制作伽马互换来在体外进行抗原。 这个反应是 肠道中的强度比周围更强。 我们还使用了这些方法 测试用自体树突状细胞免疫的猕猴 在整合酶基因中被转染和HIV-1病毒突变。 这些动物显示出类似于感染动物的反应， 我们确定，正如预期的那样，响应细胞实际上是 CD69+活化的T细胞。 另外，我们已经证明了这些动物 对HIV-1抗原具有经典的细胞毒性T淋巴细胞反应。 资助NIH授予RR00166和AI26503。