DISSEMINATION & TRAINING

传播

• 批准号: 6206618
• 负责人: ROBERT M SWEET
• 金额: $ 15.55万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206618
• 关键词: AIDS; animal tissue; bioimaging /biomedical imaging; biological products; biomedical resource; immunology; infection; inflammation; lymphatic system; radiography; radiology; structural biology; virus

To solve the three dimensional structures of multiple murine T cell receptors specific for a viral octapeptide (VSV8) bound to the K b MHC class I molecule T cells recognize cell-associated pathogens of viral, bacteria[ or fungal origin as well as tumor antigens through a unique molecule termed the T cell receptor (TCR). The TCR complex consists of multiple transmembrane chains on the surface of T lymphocytes',". The disulfide-linked a-P heterodimer is a clonally unique component that possesses a recognition site for antigen in the context of the major histocompatibility complex (MHC), while the invariant CD3 components (y, 6, e, and Q are involved in signal transduction. Because of the intimate membrane association of this complex, study of the molecular features of TCR recognition necessarily have been indirect. Recent efforts to understand the nature of this recognition process have focused on structural studies of the system. It has been known for a few years that both class I and class 11 MHC molecules, despite the very different chemical compositions of the two subunits, have the same topologic structure"-s. in particular, the way MHC molecules present the antigenic peptide is similar. A short antigenic peptide (8-10 residues for class I and >1 2 residues for class 11) lies in a large groove at the top of the MHC molecule. This groove is composed of eight strands of anti-parallel P-sheet as a floor, and two anti-parallel a-helices as side walls. In class I MHC molecules, the whole groove is formed by domain al and a2 from the same a chain; in class 11 MHC molecules, the groove is formed by domain al, and 01 from two different chains (a and 0). Structural details of how peptides bind with high affinity to these MHC molecules have been well characterized.

解决多种鼠t的三维结构 与K的病毒八肽（VSV8）相关的细胞受体 B MHC I类分子 T细胞识别病毒，细菌的细胞相关病原体[或 通过独特的分子，真菌起源以及肿瘤抗原 称为T细胞受体（TCR）。 TCR复合物由 T淋巴细胞表面上的多个跨膜链”。 二硫键链接A-P异二聚体是一种具有克隆独特的组件 在主要背景下拥有抗原的识别位置 组织相容性复合物（MHC），而不变的CD3组件 （Y，6，E和Q参与信号转导。 这种复合物研究的亲密膜关联 TCR识别的特征必定是间接的。 了解这种认可过程的性质的最新努力 专注于系统的结构研究。 众所周知 几年来，I类和11级MHC分子，尽管 两个亚基的完全不同的化学成分具有 相同的拓扑结构“ -s。特别是MHC分子的方式 存在抗原肽相似。 短抗原肽 （I级的8-10个残留物，> 1 2类残留物）位于 MHC分子顶部的大凹槽。 这个凹槽是组成的 在八股反平行的p表作为地板中，有两个 反行A螺旋作为侧壁。 在I类MHC分子中 整个凹槽由域Al和A2形成。在 11级MHC分子，凹槽由域Al形成，01从 两个不同的链（A和0）。 肽的结构细节 与这些MHC分子高亲和力结合很好 特征。