OPTICAL BIOSENSOR TO STUDY MACROMOLECULE INTERACTIONS

用于研究大分子相互作用的光学生物传感器

• 批准号: 2766461
• 负责人: William A. Cramer
• 金额: $ 23.8万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-15 至 2000-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2766461
• 关键词: biomedical equipment; biomedical equipment purchase; intermolecular interaction; surface plasmon resonance

The properties and capabilities are described of a biosensor instrument that will be used to define interactions between macromolecules. Its acquisition would establish one of an array of instruments to be used for this purpose. The proposal is submitted by seven investigators involved in studies on protein-protein, ligand-protein, peptide-protein, or protein-membrane interactions, who have expertise in high resolution protein structure studies, and in areas of biochemistry, biophysics, and molecular genetics. The biosensor instrumentation would complement and extend the information obtained from the high resolution structure studies by providing information on protein function and mechanism. A wide range of problems will be addressed. These include: (i) the nature of the membrane binding affinity of "membrane-active" proteins; (ii) the interaction between colicins and their cellular receptor; (iii) the binding affinity of antibodies to human rhinovirus; (iv) receptor interactions of enterotoxins; (v) altered binding of NAD+ by Asian alcohol dehydrogenase; (vi) binding of mitochondrial leader peptides to their receptors; (vii) interaction of G protein receptors with caveolin; (viii) interactions of erythrocyte band III peptide with glycolytic enzymes, and of lyn tyrosine kinase and B-cell antigen receptor; (ix) SH2-mediated interactions between p72 syk and the src-family kinase lck; (x) profilin-actin interactions and their modulation by ligands including proline-rich proteins and phosphoinositide lipids.

描述了将用于定义大分子之间相互作用的生物传感器仪器的特性和功能。 收购它将建立用于此目的的一系列工具之一。 该提案由七名参与蛋白质-蛋白质、配体-蛋白质、肽-蛋白质或蛋白质-膜相互作用研究的研究人员提交，他们在高分辨率蛋白质结构研究以及生物化学、生物物理学和分子遗传学领域拥有专业知识。 生物传感器仪器将通过提供有关蛋白质功能和机制的信息来补充和扩展从高分辨率结构研究中获得的信息。一系列广泛的问题将得到解决。 这些包括：（i）“膜活性”蛋白的膜结合亲和力的性质； (ii) 大肠菌素与其细胞受体之间的相互作用； (iii) 抗体与人鼻病毒的结合亲和力； (iv) 肠毒素的受体相互作用； (v) 改变亚洲醇脱氢酶对 NAD+ 的结合； (vi)线粒体前导肽与其受体的结合； (vii) G蛋白受体与小窝蛋白的相互作用； (viii)红细胞带III肽与糖酵解酶以及lyn酪氨酸激酶和B细胞抗原受体的相互作用； (ix) SH2介导的p72 syk和src家族激酶lck之间的相互作用； (x) Profilin-肌动蛋白相互作用及其通过配体的调节，包括富含脯氨酸的蛋白质和磷酸肌醇脂质。