Biacore 3000 System

Biacore 3000 系统

基本信息

批准号：
6876821
负责人：
Jannette Carey
金额：
$ 29.07万
依托单位：
PRINCETON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-04-01 至 2006-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): A commercially available instrument for quantitative surface plasmon resonance detection of macromolecular complexes and recovery of molecular components is requested. This instrument detects binding events directly through the resulting change in mass. Its presence on campus will greatly facilitate ongoing, NIH-funded basic investigations that share the fundamental long-term goal of understanding the chemical and physical principles that underlie biologically and medically relevant interactions. A wide range of systems under study here are presently hampered by the need for developing a dedicated binding assay for each one. In addition, current assays have limited dynamic range and/or prohibitive materials requirements, and yield more limited information than is available from the proposed equipment. This proposal documents how user access to the proposed instrumentation will overcome the following limitations of presently employed methods: lack of non-perturbing experimental observables that monitor binding events; prohibitively high amounts and/or concentrations of macromolecular samples; severely restricted sensitivity and dynamic range of observations; and rate-limiting throughput for multi-sample analysis. Preliminary studies are reported that demonstrate the applicability of the instrument for its intended purposes and the expertise required for its effective implementation. The instrument will also support instruction in the fundamental principles and practice of ligand-binding analysis for graduate and advanced undergraduate students.

描述（由申请人提供）：要求大分子分子复合物的定量表面等离子体共振检测和分子成分的恢复。该仪器通过产生的质量变化直接检测到结合事件。它在校园中的存在将极大地促进NIH资助的基本研究，这些调查具有理解生物学和医学相关互动构成的化学和物理原理的基本长期目标。目前，对每个系统的研究都受到了为每个系统开发专用结合测定法的广泛系统。此外，当前的测定法具有有限的动态范围和/或过度的材料要求，并且比拟议设备可获得更多的信息。该提案记录了用户对拟议仪器的访问将如何克服目前使用的方法的以下局限性：缺乏监控绑定事件的非扰动实验可观察物；大分子样品的高度和/或浓度高；严格限制的灵敏度和动态观测范围；和限速吞吐量用于多样本分析。据报道，初步研究证明了该工具的适用性以及其有效实施所需的专业知识。该工具还将支持研究生和高级本科生配体结合分析的基本原则和实践的指导。