Realtime Monitoring of Trace Amounts of Pesticides from Water Using Carbo

使用 Carbo 实时监测水中痕量农药

• 批准号: 7809943
• 负责人: SHALINI PRASAD
• 金额: $ 10万
• 依托单位: DAHL NATURAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7809943
• 关键词: Acetylcholinesterase; Acute; Address; Adolescent; Affinity; Agriculture; Air; Aldehydes; Aluminum; Amines; Animals; Antibodies; Area; Arizona; Automobile Driving; Back; Bacteria; Binding; Biochemistry; Biological; Biosensor; Birds; Brain; Budgets; Caliber; Carbaryl; Carbon; Carbon Nanotubes; Carcinogens; Cells; Cereals; Cessation of life; Chemistry; Chlorinated Hydrocarbons; Chlorpyrifos; Cholinesterases; Chronic; Communication; Complex; Computer Systems; Conflict (Psychology); Congenital Abnormality; Congresses; Country; Coupled; Culicidae; Data; Databases; Deposition; Detection; Development; Devices; Diazinon; Disputes; Dust; Economics; Electrodes; Electronics; Endosulfan; Engineering; Environment; Environmental Health; Enzymes; Equipment; Equipment Contamination; Exposure to; Fisheries; Fishes; Food; Funding; Goals; Government; Grant; Health; Human; Human Resources; Human body; Immobilization; Individual; Ingestion; Ink; Inorganic Sulfates; Insect Control; Insecta; Insecticides; Internet; Intranet; Isomerism; Ketones; Knowledge; Length; Light; Liquid substance; Location; Malathion; Marketing; Measurement; Measures; Mechanics; Memory; Metabolism; Methods; Microelectrodes; Microfluidics; Minerals; Mites; Modeling; Monitor; Movement; Mus; Nanoarray Analytical Device; Nanotechnology; Nerve; Nervous system structure; Neuraxis; Neurons; Noise; Oceans; Oregon; Organophosphates; Osteogenesis; Parasympathomimetic Agents; Performance; Pest Control; Pesticides; Phase; Plants; Plastics; Poison; Price; Principal Investigator; Printing; Public Health; Pump; Rattus; Real-Time Systems; Records; Recreation; Research; Research Personnel; Resistance; Risk; Rivers; Rodent; Running; Salmon; Sampling; Sensitivity and Specificity; Services; Severities; Signal Transduction; Soil; Solid; Solutions; Source; Specific qualifier value; Spectrum Analysis; Subgroup; Surface; Surveys; Symptoms; System; Systems Integration; Tea; Techniques; Technology; Testing; Theft; Time; Toxic effect; Training; Translating; Travel; Tube; Ultrasonography; Universities; Unspecified or Sulfate Ion Sulfates; Washington; Water; Wireless Technology; Wood material; Work; Yeasts; absorption; base; carcinogenicity; chemical bond; commercial application; commercialization; cost; cost effective; cross reactivity; design; drinking water; electric impedance; fruits and vegetables; improved; innovation; killings; malaoxon; man; member; milligram; monitoring device; multi walled carbon nanotube; nanofluidic; nanomaterials; nanoscale; nanosensors; new technology; particle; pesticide interaction; pressure; prevent; product development; programs; prototype; public health relevance; sample collection; sensor; spinal cord and brain injury; superfund site; wasting; water quality; water sampling

DESCRIPTION (provided by applicant): Development of a Field Applicable Real-time Remote Monitoring Sensor Device Capable of Detecting multiple pesticides simultaneously in fluid or surface water. We propose to use carbon nanotube matrices as the active biosensor area for the effective immobilization of the pesticides through antibody based conjugation. Our initial work has demonstrated sensitivities in the nanogram/ml regime with very low cross reactivity in the detection of the 3 pesticides, Endosulfan sulfate, Edrin aldehyde, edrin ketone.. Principal Investigator: Shalini Prasad Ph. D, Arizona State Phase I (The Sensor): This work, will utilize an ultra sensitive electrochemical sensing platform based on aligned MWCNT for voltammetric analysis of pesticides. We will examine the capability and performance of the carbon nano-array based sensing unit and develop simultaneous and continuous detection of multiple pesticides. Phase One (The System): will develop automated delivery and clearance of fluid samples to the test sensor using a proprietary design for an electronic microfluidic pump mechanism and turbine run recharging unit coupled to pneumatic mechanisms. Application of the Project to Product Development and Commercialization: Statement of the problem. Our nation's water quality is at risk since we cannot afford the man power and time it takes to sample and analyze water using current methods. The risk pesticides pose has been grossly underestimated. A recent study, of the U.S. 2009 Geological survey done by NOAA Fisheries Service and Washington State University, indicates that the combined effect of multiple pesticides is far more dangerous to the health of wildlife and humans than was estimated by analyzing the results for the effect of each pesticide individually1. In fact when juvenile west coast salmon were exposed to only two pesticides at once they immediately died. These were common pesticides used in agriculture that were thought to be safe. This lack in capability of providing water comprehensive testing for the health of the public and aquatic environments is not limited to Oregon; it is a nation wide problem. Solution: A field applicable real-time remote monitoring device to detect pesticides in water would allow DEQ, EPA and NIEH researchers to get enough data on the relationship between environmental and industrial or agricultural factors and specific mechanisms that effect water quality, to effect change. Currently there is not enough data to develop comprehensive and effective plans for managing pesticides. Dahl Natural and Arizona State are partnering to develop feasibility of this technology so that it can be commercialized to provide a solution to this health and environmental threatening problem. Commercial Applications and Other Benefits. Unique to this solution is the ability of this device to be deployed in an aquatic environment for from one to two years without the need for servicing until the end of that time period. In addition the data can be remotely sent to many different data bases at once. Hence the commercial application and benefits to the NIEH and the public are many and include waste water monitoring and alarm systems, superfund site monitoring, timely individual wastewater discharge permits issued, establishment of a comprehensive data base for NIEH, DEQ, EPA and University researchers. The market for pesticide testing is over 1 Billion dollars a year. The low cost of the sensors and dramatic cost saving per test (from $200 to $600 a day per pesticide for one mean sample to over 50 mean samples per day for as little as $30.00) as well as increase access to internet /intranet accessible data. Key Words. Field Applicable Remote Monitoring of Pesticides in Water, Electrochemical Sensor, Self-assembled MWCNT, antibody based conjugation, Endosulfan sulfate, Edrin aldehyde, edrin ketone Summary for Members of Congress. The NIEH has funded a ground breaking research effort which could dramatically improve our nation's ability to detect threats to drinking water, lakes, rivers and ocean aquatic environments. This new technology will be able to remotely monitor toxic pesticides for 1-2 years at a time. PUBLIC HEALTH RELEVANCE: The goal of this project is to integrate Dahl Natural's sampling system and wireless communication with the expertise of Dr. Shalini Prasad in nanotechnology based sensors using surface functionalized multi-walled carbon nanotube arrays (mats); and, to develop a practical, robust and cost-effective sensing system for routine monitoring of pesticides in surface waters.

描述（由申请人提供）：开发一种现场适用的实时远程监测传感器装置，能够同时检测流体或地表水中的多种农药。我们建议使用碳纳米管基质作为活性生物传感器区域，通过基于抗体的结合来有效固定农药。我们的初步工作已经证明，在检测 3 种农药（硫丹硫酸盐、埃德剂醛、埃德剂酮）时，纳克/毫升范围内的灵敏度非常低，交叉反应性非常低。 首席研究员：Shalini Prasad 博士，亚利桑那州立大学第一阶段（传感器）：这项工作将利用基于对齐 MWCNT 的超灵敏电化学传感平台进行农药伏安分析。我们将检查基于碳纳米阵列的传感单元的能力和性能，并开发同时连续检测多种农药的方法。 第一阶段（系统）：将使用电子微流体泵机构和与气动机构耦合的涡轮运行再充电单元的专有设计，开发流体样本到测试传感器的自动输送和清除。该项目在产品开发和商业化中的应用：问题的陈述。我们国家的水质正面临风险，因为我们无力承担使用现有方法采样和分析水所需的人力和时间。农药造成的风险被严重低估了。美国国家海洋和大气管理局 (NOAA) 渔业局和华盛顿州立大学对 2009 年美国地质调查进行的一项最新研究表明，多种农药的综合影响对野生动物和人类的健康造成的危害远比通过分析农药影响结果所估计的要大得多。每种农药单独1。事实上，当幼年西海岸鲑鱼同时接触两种农药时，它们立即死亡。这些是农业中常用的农药，被认为是安全的。这种缺乏为公众和水生环境健康提供水综合检测能力的情况不仅限于俄勒冈州；这是一个全国性的问题。解决方案：用于检测水中农药的现场适用的实时远程监测设备将使 DEQ、EPA 和 NIEH 研究人员能够获得有关环境与工业或农业因素之间关系以及影响水质和影响变化的具体机制的足够数据。目前没有足够的数据来制定全面有效的农药管理计划。 Dahl Natural 和亚利桑那州立大学正在合作开发这项技术的可行性，以便将其商业化，为这一威胁健康和环境的问题提供解决方案。商业应用和其他好处。该解决方案的独特之处在于该设备能够在水生环境中部署一到两年，而无需在该时间段结束之前进行维修。此外，数据可以一次远程发送到许多不同的数据库。因此，商业应用以及给 NIEH 和公众带来的好处是多方面的，包括废水监测和报警系统、超级基金现场监测、及时发放个人废水排放许可证、为 NIEH、DEQ、EPA 和大学研究人员建立综合数据库。农药检测市场每年超过10亿美元。传感器成本低廉，每次测试可大幅节省成本（从每天每种农药一个平均样品的 200 至 600 美元到每天 50 多个平均样品的成本低至 30.00 美元），以及增加对互联网/内联网可访问数据的访问。关键词。现场适用的水中农药远程监测、电化学传感器、自组装多壁碳纳米管、基于抗体的缀合、硫丹硫酸盐、艾德林醛、艾德林酮国会议员摘要。 NIEH 资助了一项突破性的研究工作，该研究工作可以极大地提高我们国家检测饮用水、湖泊、河流和海洋水生环境威胁的能力。这项新技术将能够一次远程监测有毒农药1-2年。 公共健康相关性：该项目的目标是将 Dahl Natural 的采样系统和无线通信与 Shalini Prasad 博士在使用表面功能化多壁碳纳米管阵列（垫）的基​​于纳米技术的传感器方面的专业知识相结合；开发实用、稳健且具有成本效益的传感系统，用于地表水中农药的日常监测。