Biacore 3000 shared instrument

Biacore 3000 共享仪器

• 批准号: 6876818
• 负责人: DANIEL H CONRAD
• 金额: $ 29.07万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2006-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876818
• 关键词: biomedical equipment purchase; intermolecular interaction; surface plasmon resonance

DESCRIPTION (provided by applicant): The Biacore 3000 system utilizes surface plasmon resonance technology to provide automated and sensitive technology for for label free studies of biomolecular binding. Samples ranging from small molecules to crude extracts, lipid vesicles, viruses, bacteria and eucaryotic cells can be studied. General advantages of SPR technology include being rapid, sensitive, versatile, small amount of material used, and is label-free. The only currently available instrument is a bialite upgrade that is deficient in automation, temperature range and sensitivity, even when compared to the regular BiacoreX. The users have applications which will examine protein-protein, protein-nucleic acid and protein drug interactions. The core group in this application has experience, including publications, using this technology via the use of the modified BiacoreX (bialite upgrade) instrument. The limitations of the BiacoreX include the lack of automatic sample handling, decreased sensitivity, limited temperature use range and use of only one-half of the flow cells on the sensor chip. In addition, the-3000 is designed for individual sample characterization where the highest resolution in kinetic analysis is essential and the range goes from as little as 4 RU to 70000. Upgrading to the 3000 will overcome all of these limitations, allowing much greater flexibility in experiment design and types of analyses that are possible. The instrument will be placed in the nucleic acids research core facility, run by Dr. Greg Buck, one of the core users of the instrument. A dedicated operator has been identified to run samples and assist users with data analysis. This set-up will allow much greater access to the instrument for both the core users as well as the research community as a whole. Support for the instrument operation and maintenance will be through user fees as well as university support from the cancer center and life sciences center.

描述（由申请人提供）：BIACORE 3000系统利用表面等离子体共振技术提供自动化和敏感的技术，用于用于标签的生物分子结合的免费研究。可以研究从小分子到粗提取物，脂质囊泡，病毒，细菌和胞核细胞的样品。 SPR技术的一般优势包括快速，敏感，多功能，所使用的少量材料，并且没有标签。当前唯一可用的仪器是一种双重升级，即使与常规的biacorex相比，自动化，温度范围和灵敏度不足。用户具有将检查蛋白质蛋白质，蛋白质核酸和蛋白质药物相互作用的应用。该应用程序中的核心组具有通过使用改良的biacorex（Bialite升级）仪器的经验，包括出版物。 Biacorex的局限性包括缺乏自动样品处理，敏感性降低，温度使用范围有限，并且在传感器芯片上仅使用一半的流动池。此外，3000设计用于单个样本表征，在动力学分析中最高分辨率是必不可少的，并且范围从4 ru到70000。升级到3000将克服所有这些局限性，从而使实验设计和可能的分析类型更大。该仪器将被放置在由该仪器的核心用户之一Greg Buck博士经营的核酸研究核心设施中。已经确定了专门的操作员来运行样本并协助用户进行数据分析。这种设置将为核心用户以及整个研究社区提供更大的访问权限。对仪器操作和维护的支持将是通过用户费用以及癌症中心和生命科学中心的大学支持。