FACSCALIBER

流式细胞仪

• 批准号: 6440238
• 负责人: DANIEL H CONRAD
• 金额: $ 11.45万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440238
• 关键词: biomedical equipment; biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): The Department of Microbiology and Immunology at the Medical College of Virginia Campus of Virginia Commonwealth University has maintained and operated a FACScan flow cytometer since 1988 and has used this equipment extensively (documentation attached). The instrument is now 13 years old and is in need of replacement. In addition, the replacement (FACScaliber) has an additional laser making possible four color analyses. The combination effect of heavy usage along with additional capacities, leads to this request for funds for purchase of the FACScaliber. The existing FACScan will be also kept as a backup plus overflow instrument. Usage is such that it is frequently difficult to obtain time on the instrument. The requested unit includes the 4 color cytometer, plus a Macintosh G4 computer/monitor plus Celiquest software plus peripherals for storage and print of files. User training and 2 year warranty is included. Our group consists of 10 funded investigators (NIH and one NSF) who are actively utilizing flow cytometry and have a number of publications using this technology. In addition, the unit is also available on a charge per hour basis to other users at MCV/NCU after appropriate training. At present, we have seven other laboratories that use the instrument on a less frequent basis.

描述（由申请人提供）：微生物学系和 弗吉尼亚医学院弗吉尼亚联邦分校的免疫学 大学自 1988 年以来一直维护和运行 FACScan 流式细胞仪， 已广泛使用该设备（附有文档）。该仪器是 现在13岁了，需要更换。此外，更换 (FACScaliber) 有一个额外的激光器，可以进行四色分析。这 大量使用和额外容量的结合效应，导致 此请求用于购买 FACScaliber 的资金。现有的FACScan 还将保留作为备用加溢出仪器。用法是这样的 通常很难获得使用仪器的时间。请求单位 包括 4 色细胞仪，以及 Macintosh G4 计算机/显示器 Celiquest 软件以及用于存储和打印文件的外围设备。用户 包括培训和 2 年保修。我们的团队由 10 名资助者组成 积极利用流式细胞术的研究人员（NIH 和一名 NSF） 有许多出版物使用了这项技术。此外，单位是 也可按小时收费向 MCV/NCU 的其他用户提供 适当的培训。目前，我们还有另外七个实验室使用该技术 仪器使用频率较低。