Analytical Ultracentrifuge

分析超速离心机

• 批准号: 6876390
• 负责人: ANDREW N. LANE
• 金额: $ 18.5万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6876390
• 关键词: analytical ultracentrifugation; biomedical equipment purchase; cancer registry /resource; intermolecular interaction; macromolecule; structural biology

DESCRIPTION (provided by applicant): The University of Louisville is promoting structural biology and cancer research as part of its core mission. Under the aegis of the JG Brown Cancer Center and the Department of Chemistry, a number of core facilities and major collaborative programs are being established. This currently includes NMR spectroscopy, X-ray crystallography, Molecular Modeling, and protein expression. To enable the functional analysis of macromolecules and their interactions in the solution state, we are now building a biophysics core to be headed by a senior scientist. We have identified analytical ultracentrifugation as an integral component of this core. An essential part of any functional analysis is the determination of the physical state of the isolated components (including molecular weight, shape, hydration and homogeneity), as well as measuring the stoichiometry of the complexes and the interaction strength. This is difficult to achieve by other nonequilibrium methods because of the dependent of the parameters on both shape and size. The analytical ultracentrifuge is one of the few techniques that can independently and rigorously determine mass of a functional component in a non-denaturing state in solution, as well as provide information about homogeneity and frictional properties. Furthermore, it is generally necessary to know the stoichiometry, and preferably the symmetry, of an oligomer or complex before a complete NMR structure determination can be contemplated. The analytical ultracentrifuge is an ideal shared instrument, as it can readily accommodate several research groups whose primary methodologies are not in the hydrodynamics of macromolecules, but who need the specific data it provides in order to further their research and solve problems that cannot be tackled by other techniques. Hydrodynamic analysis of macromolecules can be seen as an integral part of the overall biophysics program at the Cancer Center. The combination of structural and biophysical techniques is central to understanding regulatory processes inside cells, and for providing the basic information about basic drug targets during the drug discovery and development process. There are numerous NIH-funded investigators who need access to this instrumentation, and who would be major users. There are many other groups who will need less frequent access. All of the researchers are actively involved in protein expression and analysis of complex interactions related to disease states.

描述（由申请人提供）：路易斯维尔大学正在促进结构生物学和癌症研究，这是其核心使命的一部分。在JG Brown癌症中心和化学系的宙斯盾下，正在建立许多核心设施和主要协作计划。目前，其中包括NMR光谱，X射线晶体学，分子建模和蛋白质表达。为了实现大分子及其在溶液状态中的相互作用的功能分析，我们现在正在建立一个由高级科学家领导的生物物理学核心。我们已经确定分析超速离心是该核心的组成部分。任何功能分析的重要组成部分是确定孤立成分的物理状态（包括分子量，形状，水合和同质性），并测量复合物的化学计量和相互作用强度。由于参数在形状和大小上都取决于参数，因此难以实现其他非平衡方法。分析超级离心分析是可以独立，严格地确定溶液中非这个态状态下功能成分质量的少数技术之一，并提供了有关均匀性和摩擦特性的信息。此外，通常有必要在可以考虑完整的NMR结构确定之前了解化学计量法，最好是对寡聚或复杂的对称性。分析性超级离心分析是一种理想的共享工具，因为它可以容易地容纳几个研究小组，这些研究小组的主要方法不在大分子的流体动力学中，但是他们需要它提供的特定数据以进一步解决他们的研究并解决其他技术无法解决的问题。大分子的流体动力分析可将其视为癌症中心总体生物物理学计划不可或缺的一部分。结构和生物物理技术的组合对于理解细胞内部的调节过程以及在药物发现和发育过程中提供有关基本药物靶标的基本信息至关重要。有许多由NIH资助的调查人员需要访问此仪器，并且将成为主要用户。还有许多其他小组需要较少的访问权限。所有研究人员都积极参与与疾病状态相关的复杂相互作用的蛋白质表达和分析。