MARKER ASSISTED SELECTION OF BOVINE BLASTOCYSTS

牛胚泡的标记辅助选择

• 批准号: 6339728
• 负责人: Hans Michael Kubisch
• 金额: $ 5.71万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6339728
• 关键词: biomarker; bovine serum albumin; cell age; cow; developmental genetics; developmental immunology; early embryonic stage; embryo /fetus; embryo /fetus culture; embryo /fetus tissue /cell culture; embryo /fetus transplantation; enzyme linked immunosorbent assay; genetic transcription; genomic imprinting; genotype; in vitro fertilization; interferons

DESCRIPTION (adapted from the applicant's Abstract): The goal of this study is to investigate the possibility of using marker-assisted selection of superior embryos to increase pregnancy rates following transfer of in vitro-derived blastocysts. Such a strategy could have a significant impact on the treatment of fertility problems in humans, where pregnancy rates after in vitro fertilization and transfer of early stage embryos have remained well below 25%. An investigation of the feasibility of marker-assisted embryo selection requires a large number of embryos and recipients under controlled conditions, criteria that are unlikely to be met for human embryos. It is, therefore, proposed to use the secretion of interferon-tau (IFN-t) by bovine blastocysts as a model system to examine the hypothesis that the level of a protein produced by an individual embryo can be a useful predictor of its developmental competence. The IFN-t are Type 1 interferons that are expressed and secreted by the embryos of cattle and sheep during early pregnancy and while their precise physiological role remains unclear, there is mounting evidence implicating these interferons in the mechanism of pregnancy recognition. IFN-t can be easily detected in the culture medium of in vitro-derived bovine blastocyst, but there exists virtually no information on the significance of the observation that individual blastocysts can produce vastly different amounts of IFN-t. The aims of this proposal, therefore, are to gain further understanding of IFN-t production in embryos as well as its significance for subsequent development. Specific questions to be addressed are: 1) can culture conditions affect IFN-t production; 2) are there genotypic effects on its production; 3) does the amount of IFN-t produced by in vitro-derived blastocysts differ from that of blastocysts generated in vivo, and what is the nature of the increase in interferon-t that is found in blastocysts that are older at blastulation; 4) is IFN-t imprinted or does the amount of IFN-t that is produced by parthenogenetic and androgenetic blastocysts differ from that of control embryos, and lastly, 5) can the amount of IFN-t that is secreted in culture be a useful aid to predict the likelihood of pregnancy following transfer into recipients.

描述（改编自申请人的摘要）：本研究的目的 是为了调查使用标记辅助选择的可能性 上等胚胎以提高转移后的妊娠率 体外衍生的胚泡。 这样的策略可能会产生重大影响 关于妊娠率的人类生育问题的治疗 体外受精和早期胚胎转移后 保持低于25％。 对可行性的调查 标记辅助胚胎选择需要大量胚胎，并且 受控条件下的接收者，不太可能满足的标准 对于人类胚胎。 因此，有人建议使用 牛胚泡作为模型系统的干扰素-TAU（IFN-T）检查 假设由个体胚胎产生的蛋白质水平 可以成为其发展能力的有用预测指标。 IFN-T是 牛的胚胎表达和分泌的1型干扰素 和绵羊在怀孕初期以及它们的精确生理角色 尚不清楚，有越来越多的证据暗示这些干扰素 怀孕识别的机制。 可以轻松地在 体外牛胚泡的培养基，但存在 几乎没有关于观察的重要性的信息 单个胚泡可以产生大量不同的IFN-T。 这 因此，该建议的目的是进一步了解IFN-T 在胚胎中产生以及随后的意义 发展。 要解决的具体问题是：1）可以文化 条件影响IFN-T产生； 2）对其的基因型影响是否存在 生产; 3）是否会在体外衍生的IFN-T量 胚泡与体内产生的胚泡的不同，什么是 在胚泡中发现的干扰素-T增加的性质 吹式时期年龄较大； 4）是IFN-T印迹或IFN-T的量 这是由单骨发育和雄激发胚泡产生的 控制胚胎，最后5）可以 在文化中分泌是预测怀孕可能性的有用帮助 转移到收件人之后。