BIOFILM INTERACTIONS WITH GINGIVAL EPITHELIAL CELLS

生物膜与牙龈上皮细胞的相互作用

• 批准号: 6145846
• 负责人: Richard J Lamont
• 金额: $ 18.89万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6145846
• 关键词: Bacteroides gingivalis; bacterial cytopathogenic effect; bacterial genetics; cell adhesion; cell communication molecule; dental plaque; epithelium; gingiva; immunomodulators; molecular film; oral bacteria; oral mucosa; tissue /cell culture; virulence

Dental plaque develops as a complex biofilm on the tooth surface and is a direct precursor of periodontal diseases, one of the most common bacterial infections in developed countries. The molecular and cellular events that cause the transformation of commensal plaque into a pathogenic entity are only partially understood and involve colonization by pathogenic organisms such as Porphyromonas gingivalis which them impinge upon the structural integrity of the periodontal tissues and disrupt the host immune response. Recently, there have been two major conceptual breakthroughs in our perception of the pathogenic process. The first is that bacteria in biofilm such as plaque are phenotypically distinct from their planktonic counterparts and these phenotypic changes have important implications for virulence. Secondly, epithelial cells that line mucosal surfaces are no longer simply regarded as a mechanical barrier, but instead function as sensors for microbial infection., generating and transmitting signals among bacteria and host immune cells. The goals of this project are to investigate the molecular cross-talk between biofilm mode P. gingivalis and gingival epithelial cells. Thus, the production of immune mediators and effector molecules of tissue destruction by gingival epithelial cells, and the signal transduction pathways that participate in these responses, following contact with biofilm-P. gingivalis in the context of a biofilm will be identified. The long term goal of the project is thus to identify bacterial and gingival epithelial cell molecules or signaling pathways that could sere as targets for novel therapeutic agents to prevent or reduce the severity of periodontal diseases. As children rarely develop periodontal disease, the preventive intervention with these new technologies in at risk children could significantly improve adult periodontal health.

牙齿在牙齿表面上以复杂的生物膜形式发展，是牙周疾病的直接前体，牙周疾病是发达国家最常见的细菌感染之一。导致共生斑块转化为致病实体的分子和细胞事件仅被部分理解，并涉及诸如卟啉念珠菌等致病生物殖民化，它们会影响周期性组织的结构完整性并破坏宿主免疫反应。最近，在我们对病原过程的看法中，已经有两个主要的概念突破。首先是生物膜中的细菌在表型上与它们的浮游物质截然不同，而这些表型变化对毒力具有重要意义。其次，线粘膜表面的上皮细胞不再仅仅被视为机械屏障，而是充当微生物感染的传感器。在细菌和宿主免疫细胞之间产生和传播信号。 该项目的目标是研究生物膜模式P.牙龈和牙龈上皮细胞之间的分子串扰。因此，在与生物膜-P接触后，牙龈上皮细胞对组织破坏的免疫介质和效应分子的产生以及参与这些反应的信号转导途径。将在生物膜的背景下确定牙龈。因此，该项目的长期目标是鉴定细菌和牙龈上皮细胞分子或信号传导途径，这些分子可以作为新型治疗剂的靶标，以防止或减少牙周疾病的严重程度。由于儿童很少患上牙周疾病，因此对这些新技术的预防性干预可能会显着改善成人牙周健康状况。