ION CURRENT ANALYSIS IN THE CORNEA

角膜中的离子电流分析

• 批准号: 6073746
• 负责人: Larry S. Liebovitch
• 金额: $ 10.99万
• 依托单位: FLORIDA ATLANTIC UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6073746
• 关键词: aging; artificial intelligence; computer data analysis; computer program /software; cornea disorder; corneal endothelium; electrophysiology; eye injury; ion transport; laboratory rabbit; mathematical model; membrane channels; membrane transport proteins; model design /development; molecular biology; molecular dynamics; personal computers; tissue /cell culture; voltage /patch clamp

The transport system of the corneal endothelium maintains the cornea at the low level of hydration required for transparency and good vision. If this transport system does not function properly then permanent corneal edema, loss of transparency, and eventual blindness may occur. Our long term objective is to understand how this transport system functions under normal conditions and how it changes in aged, injured, and diseased corneas. Ion channel proteins in the cell membrane are an essential component of this transport system. We will study the dynamics of how these proteins function as "molecular machines". The specific aims for this project period are: 1) to perform patch clamp experiments to measure and compare the properties of ion channels from freshly excised and cultured corneal endothelial cells, 2) to develop and apply new mathematical methods, including powerful new methods of fractals and nonlinear dynamics (chaos) to analyze this data, and 3) to determine the properties of different types of dynamical models to understand the molecular mechanisms responsible for the channel properties.

角膜内皮的运输系统将角膜保持在 透明度和良好视力所需的水平低水平。如果 该运输系统无法正常运行，然后永久角膜 水肿，透明度的丧失和最终可能发生失明。 我们的长期目标是了解该运输系统如何 在正常条件下的功能及其在老年，受伤和 患病的角膜。 细胞膜中的离子通道蛋白是 这个运输系统。我们将研究这些蛋白质的动力学 功能充当“分子机”。 该项目期间的具体目的是： 1）执行斑块夹实验以测量和比较 来自新鲜切除和培养的角膜的离子通道的性质 内皮细胞， 2）开发和应用新的数学方法，包括强大的新方法 分形和非线性动力学（混乱）分析这些数据的方法 和 3）确定不同类型的动态模型的特性 了解负责通道的分子机制 特性。

项目成果

Statistical properties predicted by the ball and chain model of channel inactivation.

• DOI: 10.1016/s0006-3495(92)81732-0
• 发表时间: 1992-12
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: L. Liebovitch;L. Y. Selector;Richard P. Klinet

Testing fractal and Markov models of ion channel kinetics.

测试离子通道动力学的分形和马尔可夫模型。

• DOI: 10.1016/s0006-3495(89)82815-2
• 发表时间: 1989
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Liebovitch,LS

A model of ion channel kinetics based on deterministic, chaotic motion in a potential with two local minima.

基于具有两个局部最小值的电势中的确定性混沌运动的离子通道动力学模型。

• DOI: 10.1007/bf02368171
• 发表时间: 1992
• 期刊: Annals of biomedical engineering
• 影响因子: 3.8
• 作者: Liebovitch,LS;Czegledy,FP
• 通讯作者: Czegledy,FP

Analysis of fractal ion channel gating kinetics: kinetic rates, energy levels, and activation energies.

分形离子通道门控动力学分析：动力学速率、能级和活化能。

• DOI: 10.1016/0025-5564(89)90015-1
• 发表时间: 1989
• 期刊: Mathematical biosciences
• 影响因子: 4.3
• 作者: Liebovitch,LS

The akaike information criterion (AIC) is not a sufficient condition to determine the number of ion channel states from single channel recordings.

akaike 信息准则 (AIC) 不是确定单通道记录中离子通道状态数量的充分条件。

• DOI: 10.1002/syn.890050207
• 发表时间: 1990
• 期刊: Synapse (New York, N.Y.)
• 作者: Liebovitch,LS;Toth,TI
• 通讯作者: Toth,TI