ION CURRENT ANALYSIS IN THE CORNEA

角膜中的离子电流分析

• 批准号: 2159816
• 负责人: Larry S. Liebovitch
• 金额: $ 16.93万
• 依托单位: FLORIDA ATLANTIC UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2159816
• 关键词: aging; artificial intelligence; computer data analysis; computer program /software; cornea disorder; corneal endothelium; electrophysiology; eye injury; ion transport; laboratory rabbit; mathematical model; membrane channels; membrane transport proteins; model design /development; molecular biology; molecular dynamics; tissue /cell culture; voltage /patch clamp

The transport system of the corneal endothelium maintains the cornea at the low level of hydration required for transparency and good vision. If this transport system does not function properly then permanent corneal edema, loss of transparency, and eventual blindness may occur. Our long term objective is to understand how this transport system functions under normal conditions and how it changes in aged, injured, and diseased corneas. Ion channel proteins in the cell membrane are an essential component of this transport system. We will study the dynamics of how these proteins function as "molecular machines". The specific aims for this project period are: 1) to perform patch clamp experiments to measure and compare the properties of ion channels from freshly excised and cultured corneal endothelial cells, 2) to develop and apply new mathematical methods, including powerful new methods of fractals and nonlinear dynamics (chaos) to analyze this data, and 3) to determine the properties of different types of dynamical models to understand the molecular mechanisms responsible for the channel properties.

角膜内皮的运输系统使角膜保持在 透明度和良好视力所需的水分含量较低。如果 该运输系统无法正常运作，然后永久角膜 可能会发生水肿、透明度丧失，甚至最终失明。 我们的长期目标是了解这个交通系统如何 正常情况下的功能以及在衰老、受伤和受伤时如何变化 患病的角膜。 细胞膜中的离子通道蛋白是细胞膜的重要组成部分 这个交通系统。我们将研究这些蛋白质如何动态变化 发挥“分子机器”的作用。 本项目期间的具体目标是： 1）进行膜片钳实验来测量和比较 新鲜切除和培养的角膜离子通道的特性 内皮细胞， 2）开发和应用新的数学方法，包括强大的新方法 分形和非线性动力学（混沌）方法来分析这些数据， 和 3）确定不同类型动力学模型的属性 了解负责通道的分子机制 特性。