ION CURRENT ANALYSIS IN THE CORNEA

角膜中的离子电流分析

• 批准号: 3262319
• 负责人: Larry S. Liebovitch
• 金额: $ 13.31万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3262319
• 关键词: analytical method; chemical hydration; chemical models; chemical reaction; computer simulation; conformation; corneal endothelium; corneal stroma; electrophysiology; eye bank /preservation; ionophores; laboratory rabbit; mathematical model; mathematics; membrane channels; membrane permeability; voltage /patch clamp

The corneal endothelium maintains the cornea at the low level of hydration required for transparency and good vision. The long term goals are to understand how the corneal endothelium: 1) functions under normal physiological conditions, 2) how it is compromised by disease, and 3) how it can be better maintained in vitro to improve corneal storage techniques to make more corneas available for transplant. The corneal endothelium maintains the low level of hydration by fluid movements generated by the flow of ions. Ion channels in the cell membrane play a crucial role in the control of these ion movements. We will study the structure and function of these ion channels. The specific aims for this project period are to: 1) use the patch clamp technique to study and compare the properties of ion channels in fresh, stored, and cultured corneal endothelial cells, under different storage, media, voltage, ionic, temperature and other conditions. 2) develop, test, and use new mathematical techniques to analyze that data, including the powerful new methods of non-linear dynamics and chaos, and 3) use models of different kinetic and molecular mechanisms that will be compared to the experimental results to uncover the molecular mechanisms responsible for the observed channel properties.

角膜内皮使角膜维持在低水平 透明度和良好视力所需的水合作用。 长期目标 了解角膜内皮如何： 1）在正常生理条件下发挥作用， 2）它如何受到疾病的损害，以及 3）如何在体外更好地维持，以改善角膜储存 使更多角膜可用于移植的技术。 角膜内皮通过液体维持低水平的水合作用 离子流动产生的运动。 细胞内的离子通道 膜在控制这些离子运动中发挥着至关重要的作用。 我们 将研究这些离子通道的结构和功能。 该项目期间的具体目标是： 1）使用膜片钳技术来研究和比较 新鲜、保存和培养的角膜内皮细胞中的离子通道， 在不同的存储、介质、电压、离子、温度等条件下 状况。 2）开发、测试和使用新的数学技术来分析 数据，包括强大的非线性动力学新方法和 混乱，以及 3）使用不同的动力学和分子机制模型 与实验结果进行比较，揭示分子机制 负责观察到的通道属性。