PROTEIN SEQUENCER SYSTEM

蛋白质测序仪系统

• 批准号: 3521395
• 负责人: EDWARD BRESNICK
• 金额: $ 11万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521395
• 关键词: aminoacid analyzer; biomedical equipment purchase; protein purification; protein sequence; synthetic peptide

This Shared Instrumentation Grant Application is for funds to purchase a peptide sequencer for a core laboratory at Dartmouth College and Dartmouth Medical School. The Protein Core Laboratory already contains a peptide synthesizer. Currently, all proteins must be forwarded to another university or industrial concern for sequencing which is expensive and not as efficient as if Dartmouth has its own instrument. A major user group consisting of nationally-funded investigators from the Departments of Biology, Biochemistry, Chemistry, Internal Medicine, Microbiology, Pharmacology and Toxicology, and Physiology has been identified. An Advisory Committee consisting of Drs. Bresnick, Ciardelli and Froehner has been appointed to provide groundrules for the use of the facility, to establish fees for service, and to assist when needed in priority setting for individual tasks (if asked by the Facility Director). The Advisory Committee has appointed Dr. Tom Ciardelli who will have the overall day-to-day responsibility for the protein sequencing service, will oversee the technician, and will establish priorities for proteins to be sequenced. The acquisition of the peptide sequencer will significantly improve the research posture of a group of peer-reviewed investigators and will accordingly, fill a void which is existent on the Dartmouth campus.

此共享仪器拨款申请用于资金购买 达特茅斯学院核心实验室的肽测序仪和 达特茅斯医学院。 蛋白质核心实验室已经包含 肽合成仪。 目前，所有蛋白质都必须转发至 另一所大学或工业界对测序的关注是 昂贵且效率不如达特茅斯拥有自己的仪器。 一个 主要用户组由国家资助的研究人员组成 生物学系、生物化学系、化学系、内科系、 微生物学、药理学和毒理学以及生理学 确定。 由博士组成的咨询委员会。恰尔代利·布雷斯尼克 Froehner 已被任命为使用提供基本规则 设施，确定服务费用，并在需要时提供协助 个别任务的优先级设置（如果设施要求） 导演）。 咨询委员会任命 Tom Ciardelli 博士为 将全面负责蛋白质的日常工作 测序服务，将监督技术人员，并将建立 要测序的蛋白质的优先级。 肽的获取 测序仪将显着改善一组研究人员的研究状况 同行评审的研究人员，因此将填补一个空白 存在于达特茅斯校园。